Molecular phylogenetic study on genes related to sperm packets in the Volvocaceae (Chlorophyta)

沃尔沃科（绿藻）精子包相关基因的分子系统发育研究

基本信息

批准号：
05640800
负责人：
NOZAKI Hisayoshi
金额：
$ 1.34万
依托单位：
The University of Tokyo (1995)National Institute for Environmental Studies (1993-1994)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05640800/
关键词：
Genes Volvocaceae Evolutionary phylogeny Sperm packets Molecular biology Green algae

项目摘要

In order to analyze the phylogenetic position of evolution of sperm packets in the colonial families Volvocaceae and Goniaceae, phylogenetic relationships within these families were inferred based on the cladistic analysis of the morphological data. The strict consensus tree of this analysis indicated that sperm packets evolved in a Yamagishiella-like isogamous alga to give rise to a monophyletic group comprising the anisogamous/oogamous genera Eudorina, Platydorina, Pleodorina and Volvox. Furthermore, phylogenetic analysis based on the rbcL gene sequence data suggested, with high bootstrap values, the presence of a monophyletic group composed of almost all the members of the anisogamous/oogamous volvocacean species, in which Eudorina elegans was resolved as the most primitive species. It is, therefore, suggested that the genes related to the sperm packets of E.elegans is most primitive within the anisogamous/oogamous group. Thus is may be fruitful to compare such genes of Eudorina and homologous genes of Yamagishiella in order to study the evolution of genes related to sperm packets.As the first step to study the evolution of Eudorina genes related to sperm packets from genes in a Yamagishiella-like alga, isolation of genes related to sperm packets in E.elegans based on differential screening methods seemed to be essential. Therefore, methods to induce sperm packets in a large volume of E.elegans culture were established. Approximately 20% of cultured cells were induced to develop into sperm packets by using an incubator (25ﾟC) and a large flask containing Pleodorina mating medium (Nozaki et al., Phycologia 28 : 252-267,1989) continuously aerated. Comparative analyzes of mRNA extracted from the induced sperm packets and vegetative cells will resolve the phylogenetic characteristics of the genes related to sperm packets.

为了分析精子数据包在殖民地科和贡氏菌科中的系统发育位置，根据形态学数据的cladisisical分析，推断了这些家族内的系统发育关系。该分析的严格共识树表明，精子数据包在类似Yamagishiella的异构藻类中进化出来，产生一个单系组，完成了各向异性/卵形属Eudorina，Platydorina，platydorina，pleodorina，pleodorina和volvox。此外，基于RBCL基因序列数据的系统发育分析表明，具有较高的自举值，即几乎所有由各向异性/斑点的Volvocacean物种组成的单系组，其中Eudorina Elegrans被解析为最原始的物种。因此，提出与eelegans的精子数据包相关的基因是各向异性/卵形群中最原始的。比较Yamagishiella的Eudorina和同源基因的这种基因，以研究与精子数据包相关的基因的演变。因此，建立了在大量的e硫化培养物中诱导精子包的方法。诱导约20％的培养细胞通过使用孵化器（25 c）和含有胸膜pleodorina交配培养基的大烧瓶（Nozaki等人，Phycologia，Phycologia 28：252-267，1989）连续充气而发展为精子包。从诱导的精子数据包和营养细胞中提取的mRNA的比较分析将解决与精子数据包相关的基因的系统发育特征。