An analysis on oncogene mutations in oral leukoplakia with PCR-SSCP

PCR-SSCP分析口腔白斑癌基因突变

• 批准号: 03454465
• 负责人: ISHIDA Takeshi
• 金额: $ 4.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454465/
• 关键词: Tongue; Squamous cell carcinoma; Leukoplakia; p53; Gene mutations; DNA増幅; PCR

The role of p53 tumor-suppressor gene in the carcinogenesis of oral squamous cell carcinoma is still unclear. The purpose of this research was to investigate the frequencies of p53 gene mutations in oral squamous cell carcinoma and leukoplakia by means of PCR-SSCP.The template DNA was extracted from formalin-fixed, paraffin-embedded specimens (11 cases of tongue squamous cell carcinoma, 9 of tongue leukoplakia and 2 normal gingiva) by Proteinase K digestion method. PCR was performed on each sample with 4 primer with pairs for exon 5,6,7 and 8 of p53 gene. PCR products were electrophoresed in native gradient polyacrylamide gel and detected by silver staining (PCR-SSCP). Extra bands indicating the presence of mutations were detected in 1 case of squamous cell carcinoma and 1 case of leukoplakia.

p53抑癌基因在口腔鳞状细胞癌发生过程中的作用尚不清楚。本研究的目的是通过PCR-SSCP研究p53基因在口腔鳞状细胞癌和白斑中的突变频率。模板DNA从福尔马林固定、石蜡包埋的标本（11例舌鳞状细胞癌）中提取。 、 9 例舌白斑和 2 例正常牙龈）采用蛋白酶 K 消化法。使用4对p53基因外显子5、6、7和8的引物对每个样品进行PCR。 PCR 产物在天然梯度聚丙烯酰胺凝胶中进行电泳，并通过银染（PCR-SSCP）进行检测。在 1 例鳞状细胞癌和 1 例白斑病中检测到表明存在突变的额外条带。