Basic study to establish artificial lung with metabolic functions

建立具有代谢功能的人工肺的基础研究

• 批准号: 03454234
• 负责人: KITAMURA Satoshi
• 金额: $ 3.97万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454234/
• 关键词: Artifioal lnng; Arachidonic acid; Alveolar macrophage; Endothelial cell; Leukotriene; Prostaglandin; Thromboxane; Endotheline

Basic experiments were performed about alveolar macrophages and endothelial cells for these two years.Arachidonic acid metabolism of alveolar macrophages was analyzed by guinea pig alveolar macrophages. Macrophages were obtained by bronchoalveolar lavage, and incubated with arachidonic acid and A23187 for 5 minutes. Metabolites of supernatant were analyzed by reverse phase high-performance liquid chromatography. Leukotriene B_4, 5-HETE, and HHT were detected and the production of thromboxane A_2 was also speculated. Because these metabolites have very high chemotactic and bronchoconstrictive activities, it may be suggested that the metabiolites of arachidonic acid are very important to maintain the homeostasis of pulmonary function.On the other hand, metabolic functions of cultured endothelial cells were analyzed under the influence of leukotrienes, nicotine, and elastase. Leukotriene B_4 and D_4 stimulated the release of prostaglandin I_2 and thromboxane B_2 from endothelial cells. Nicotine inhibited the release of prostaglandin I_2, thromboxane B_2 and endotheline from endothelial cells in higher concentration. Elastase inhibited the permeability of cultured monolayer of endothelial cells. These results demonstrate that endothelial cells can regulate not only tonus of smooth muscle of airway and blood vessels but also chemotactic activities through the release of various chemical mediators.These basic data will provide us some basic information to establish an artificial lung with metabolic function.

在这两年中，对肺泡巨噬细胞和内皮细胞进行了基本实验。通过豚鼠猪肺泡巨噬细胞分析了肺泡巨噬细胞的芳族酸代谢。通过支气管肺泡灌洗获得巨噬细胞，并与花生四烯酸和A23187孵育5分钟。通过反相高性能液相色谱分析上清液的代谢产物。检测到白细胞B_4、5-HETE和HHT，并推测了血栓烷A_2的产生。由于这些代谢产物具有非常高的趋化性和支气管收缩活性，因此可以提出，在维持肺功能的稳态非常重要。另一方面，在Leukotrienes，Nicotrienes，Nicotine，Nicotine和Elastase和Elastase和Elastase的影响下分析了培养的内皮细胞的代谢功能。白细胞B_4和D_4刺激了从内皮细胞中释放前列腺素I_2和势力箱B_2。尼古丁抑制了前列腺素I_2，血栓烷B_2和内皮细胞中内皮细胞的释放。弹性酶抑制了内皮细胞培养的单层的渗透性。这些结果表明，内皮细胞不仅可以通过释放各种化学介质的释放来调节气道和血管平滑肌的趋化肌肉。这些基本数据将为我们提供一些基本信息，以建立具有代谢功能的人造肺。