Mechnism of anti-atherogenic action of Eicosapentaenoic acid(EPA).

二十碳五烯酸（EPA）的抗动脉粥样硬化作用机制。

• 批准号: 02454505
• 负责人: TAMURA Yasushi
• 金额: $ 3.84万
• 依托单位: Chiba University Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454505/
• 关键词: Eicosapentaenoic acid; Cholesterol; Macrophage; Oxidative-LDL; Prostacycline; Lipid peroxides; シクロオキシゲナーゼ; 抗酸化剤; 動脈硬化; 多価不飽和脂肪酸; エイユサペンタエン酸; 泡沫細胞; マクロファ-ジ; 抗酸化作用; 単球; コレステロ-ルエステル; アセチルLDL; 魚脂

1)Inhibition of foam cell formation by EPA Accumulation of cholesterol was sigunificantly decreased in EPA-rich peritoneal macrophages obtanined from rats fed with purified EPA-E. In addition receptor numbers of acetylated LDL and PDGF production were decreased in EPA-rich rat pineal macrophages. 2)Suppresion of oxidative modification of EPA-rich rabbit LDL. EPA-rich LDL obtained from rabbits fed with purified EPA-E showed a lesser susceptibility to Ca^<++>-catalysed oxidative modification. 3)Study of the effect of EPA on cells enriched with EPA. EPA suppressed proliferation of cultured rat vascular smooth muscle cell enriched with EPA. DHA had a similar effect, but lesser than EPA. 4)Effect of PGI_2 production in EPA-rich rat vascular smooth muscle cells. Enhanced PGI_2 synthesis was noted in EPA-enriched rat vascular smooth muscle cells. This could be most likely explained by stimulation of cyclooxygenase activity by lipid peroxides derived from EPA.

1) EPA 抑制泡沫细胞形成 从喂食纯化 EPA-E 的大鼠中获得的富含 EPA 的腹膜巨噬细胞中，胆固醇的积累显着减少。此外，富含 EPA 的大鼠松果体巨噬细胞中乙酰化 LDL 和 PDGF 生成的受体数量减少。 2）抑制富含EPA的兔低密度脂蛋白的氧化修饰。从喂食纯化EPA-E的兔子获得的富含EPA的LDL显示出对Ca 2+ 催化的氧化修饰的敏感性较低。 3)研究EPA对富含EPA的细胞的影响。 EPA 抑制富含 EPA 的培养大鼠血管平滑肌细胞的增殖。 DHA 也有类似的作用，但比 EPA 弱。 4)富含EPA的大鼠血管平滑肌细胞中PGI_2产生的影响。在富含 EPA 的大鼠血管平滑肌细胞中发现 PGI_2 合成增强。这很可能是通过 EPA 衍生的脂质过氧化物刺激环氧合酶活性来解释的。

项目成果

Tamura Y Hiroi A,Terano T Saitoh H and Yoshida S: "Anti-Atherogenic and anti-thrombotlc aitin of EPA" Proeeding M Symposium of w-3 FattY acids Nutabolism and Biolosial Effects. (1993)

Tamura Y Hiroi A、Terano T Saitoh H 和 Yoshida S：“EPA 的抗动脉粥样硬化和抗血栓艾汀”W-3 脂肪酸营养代谢和生物损失效应 M 研讨会论文集。

斉藤 博幸,田村 泰: "Fishoilの血清脂質低下作用" 循環器科. 29. 379-385 (1991)

Hiroyuki Saito、Yasushi Tamura：“鱼油的血清脂质降低作用”心脏病学 29. 379-385 (1991)

Hirai A,Terano T,Tamura Y.: "Current Topics in Nutritin and Direares(Omega-3tatty acids Cpidelaiogical and Climial As peis" GA.Sbiller and J.Scala Alan R.Lis Inc.N.Y., 229-252 (1990)

Hirai A、Terano T、Tamura Y.：“Nutritin 和 Direares（Omega-3tatty 酸 Cpidelaiogical 和 Climial As peis）的当前主题”GA.Sbiller 和 J.Scala Alan R.Lis Inc.N.Y.，229-252 (1990)

Tamura Y, Hirai A, Terano T, Saitoh H and Yoshida S.: "Anti-atherogenic and anti-thrombotic action of EPA" Proceeding of the 5th Scientific Meeting of the Society for Research on PUFAs. (1993)

Tamura Y、Hirai A、Terano T、Saitoh H 和 Yoshida S.：“EPA 的抗动脉粥样硬化和抗血栓作用”多不饱和脂肪酸研究学会第五届科学会议论文集。

Chang Kj,Saitoh H.,Tasuno J.,Tamura Y.,Yoshida S.Ozawa A,Fujita T.: "Effect of EPA on the binding and the drardation of oxidized LDL and the production of LTB_4,LTC_4 and PGE_2 in rat pcritoncal macrophages" Annual.Review of New York acudrmy of Samce. 491

Chang Kj、Saitoh H.、Tasuno J.、Tamura Y.、Yoshida S.Ozawa A、Fujita T.：“EPA 对大鼠胰腺中氧化 LDL 的结合和沉积以及 LTB_4、LTC_4 和 PGE_2 产生的影响