Molecular and Biological Study on Roles of Colony-Stimulating Factors in the Formation of Granulomatous Lung Lesions.

集落刺激因子在肉芽肿性肺病变形成中作用的分子和生物学研究。

• 批准号: 02454238
• 负责人: YAMAGUCHI Etsuro
• 金额: $ 4.1万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454238/
• 关键词: Granulocyte-macrophage colony-stimulating factor (GM-CSF); Interleukin-3 (IL-3); Macrophage colony-stimulating factor (M-CSF); Reverse transcription-polymerase chain reaction (RT-PCR); Sarcoidosis; Bronchoalveolar lavage (BAL); マクロファ-ジコロニ-刺激因子; 逆転写

Pulmonary sarcoidosis is characterized by the accumulation of a great number of alveolar macrophages at local milieu of the lung. In order to investigate its mechanism, We aimed to examine the expression of mRNA of several colony-stimulating factors (CSFs). We extracted RNA from inflammatory cells obtained by bronchoalveolar lavage, which were composed mainly of alveolar macrophages and lymphocytes. mRNA of interest was detected by reverse transcription-polymerase chain reaction (RT-PCR) method using synthesized primers of macrophage-CSF (M-CSF), interleukin-3 (IL-3), and granulocytemacrophage-CSF (GM-CSF).M-CSF mRNA was detected in all normal subjects, patients with pulmonary sarcoidosis, and patients with farmer's lung disease. Meanwhile, IL-3 mRNA was detected none of them. GM-CSF mRNA was detected in 15 of 20 patients with pulmonary sarcoidosis, whereas it was expressed in none of normal subjects and patients with farmer's lung disease. GM-CSF is assumed to contribute the accumulat … More ion of alveolar macrophages by activation, differentiation, and proliferation of monocytes. As it also has a fusing effect of alveolar macrophages, it may be involved in the formation of multinucleated giant cells within the granulomas.It is noteworthy that results of the expression of GM-CSF mRNA was contrasting between pulmonary sarcoidosis and farmer's lung disease, suggesting different pathogenetic mechanisms are working in these two granulomatous lung diseases.We also examined the relationship between the expression of GM-CSF mRNA and clinical significance in pulmonary sarcoidosis. Patients with mRNA expression had significantly higher proportion of bronchoalveolar lavage lymphocytes, CD4/8 ratio, and serum ACE levels than those without the expression. Retrospective study from the time when mRNA was examined revealed association between mRNA expression and unchanged or worse clinical course judged by chest X-ray films. Prospective study gave similar results, thus demonstrating substantial role of GM-CSF in the pathogenetic mechanism of pulmonary sarcoidosis. Less

肺结节病的特征是在肺部局部环境中积累了大量肺泡巨噬细胞。为了研究其机制，我们旨在检查几个刺激因子（CSF）的mRNA的表达。我们从通过支气管肺泡灌洗获得的炎性细胞中提取了RNA，这些RNA主要由肺泡巨噬细胞和淋巴细胞组成。使用巨噬细胞-CSF（M-CSF）的合成引物（M-CSF），肠介毒素3（IL-3）和粒细胞嗜血杆菌-CSF（GM-CSF）。平均情况，未检测到IL-3 mRNA。在20例肺结节病患者中，有15例检测到GM-CSF mRNA，而在任何正常受试者和农民肺部疾病的患者中都没有表达。 GM-CSF被认为可以通过激活，分化和单核细胞增殖来促进肺泡巨噬细胞的更多离子。由于它还具有肺泡巨噬细胞的融合作用，因此它可能与颗粒中多核巨细胞的形成有关。值得注意的是，GM-CSF mRNA表达的结果在肺结节病和农民的肺部疾病之间形成鲜明对比，这表明这两种肉芽肿性肺部疾病正在起作用。 MRNA表达的患者比没有表达的患者的支气管肺泡灌洗淋巴细胞，CD4/8比例和血清ACE水平明显更高。回顾性研究从检查mRNA的时间，发现mRNA表达与胸部X射线膜判断的不变或较差的临床过程之间的关联。前瞻性研究给出了相似的结果，因此证明了GM-CSF在肺结节病的致病机制中的重要作用。较少的

项目成果

Etsuro Yamaguchi, Yoshikazu Kawakami: "What can we know by BAL?-from DNA level-" Bronchology. 14. (1992)

Etsuro Yamaguchi、Yoshikazu Kawakami：“我们可以通过 BAL 了解什么？-从 DNA 水平-”支气管学。

伊藤 昭英,山口 悦郎,古家 乾,桧沢 伸之,阿部 庄作,川上 義和: "サルコイド-シス患者BAL細胞によるGMーCSFmRNA発現とその臨床的意義" サルコイド-シス学会雑誌. 10. 93-94 (1991)

Akihide Ito、Etsuro Yamaguchi、Inui Furuya、Nobuyuki Hizawa、Shosaku Abe、Yoshikazu Kawakami：“结节病患者 BAL 细胞的 GM-CSF mRNA 表达及其临床意义”结节病学会杂志 10. 93-94（1991 年）。 ）

Akihide Itoh, Etsuro Yamaguchi, Ken Furuya, Nobuyuki Hizawa, Shosaku Abe, Yoshikazu Kawakami: "Expression of GM-CSF mRNA by BAL cells from sarcoid patients and its clinical significance." Jpn J Sarcoidosis. 10. 93-94 (1991)

Akihide Itoh、Etsuro Yamaguchi、Ken Furuya、Nobuyuki Hizawa、Shosaku Abe、Yoshikazu Kawakami：“来自结节病患者的 BAL 细胞的 GM-CSF mRNA 表达及其临床意义。”

Itoh A,Yamaguchi E,Kuzunoki N,Okazaki N,Furuya K,Abe S,Kawakami T.: "Expression of granulocyte-macrophage colony-stimulating factor mRNA by inflammatory cells in the sarcoed lung." An J Respin Cell Mol Biol. 3. 245-249 (1990)

Itoh A、Yamaguchi E、Kuzunoki N、Okazaki N、Furuya K、Abe S、Kawakami T.：“肉瘤肺中炎症细胞表达粒细胞巨噬细胞集落刺激因子 mRNA。”

伊藤 昭英,山口 悦郎,古家 乾,桧沢 伸之,阿部 庄ご,川上 義和: "サルコイド-シス患者BAL細胞によるGMーCSFmRNA発現とその臨床的意義" サルコイド-シス学会雑誌. 10. 93-94 (1991)

Akihide Ito、Etsuro Yamaguchi、Inui Furuya、Nobuyuki Hizawa、Shogo Abe、Yoshikazu Kawakami：“结节病患者 BAL 细胞的 GM-CSF mRNA 表达及其临床意义”结节病学会杂志 10. 93-94（1991 年）。 ) )