Studies on the Interaction between Plant Secondary Metabolites and Xenobiotics

植物次生代谢产物与外源物质相互作用的研究

基本信息

批准号：
02454064
负责人：
TAHARA Satoshi
金额：
$ 3.78万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

Biological and chemical studies on the interaction between plant seconda secondary metabolites and xenobiotics were conducted to reveal the potency of protective facilities of higher plants. 1. At first the plant secondary metabolites which exhibited antidoting activity against benzimidazole fungicides were surveyed and the following five compounds all from Polygonaceae were recognized as anti-MBC (=carbendazim, an active principle of benomyl and thiophanate-methyl both benzimidazole type fungicides) substances, and identified their chemical structures: emodin (1) and alpha-tocopherol(2)from Polygonum sachalinense; 5-methoxy-6,7-methylene-dioxyflavone (3) and 3,5-dihydroxy-4-methylstilbene (4) from P. nodosum; and 2,6-dimethoxy-1,4-benzoquinone (5) from P. thunbergii. 2. Structure-activity relationships between the antidoting activity against MBC and quinones (anthra- and naphtho-quinones) or flavones were analyzed. The most active compounds were lapachol (3-hydroxy-4-prenylnaphthoquinone) in quinones and unsubstituted flavone or 5-methoxyflavone in flavonoids. 3. The mode of action of the antidoting agent against MBC which is believed to be an inhibitor of tubulin polymerization and consequently resulting in a failure of mitosis, was very much interested. So far as we know at present is that the antidotes mentioned above do not compete with MBC for the binding site of beta-tubulin in vitro. 4. Some physiologically active secondary metabolites, isoflavonoids, sesquiterpene derivatives and oligostilbenes were isolated and elucidated their structures respectively, from Piscidia erythrina (Leguminosae); Rosa rugosa (Rosaceae) and Chloranthus Japonicus and C. serratus (Chloranthaceae); and Carex spp. (Cypreaceae).

进行了有关植物二次代谢物与异种生物学之间相互作用的生物学和化学研究，以揭示高等植物保护设施的效力。 1。首先，对植物次生代谢产物对苯唑唑杀菌剂的抗毒活性进行了调查，以下五种化合物全部都被认为是抗MBC（= carbendazim（= Carbendazim）（carbendazim），并鉴定出它们的化学结构：来自多边形Sachalinense的Emodin（1）和α-生育酚（2）； 5-甲氧基-6,7-甲基 - 二氧化氟氟氟氟此（3）和3,5-二羟基-4-甲基苯乙烯（4），来自P. nodosum；和2,6-二甲氧基-1,4-苯喹酮（5），来自P. thunbergii。 2。分析了针对MBC和Quinones（Anthra和Naphtho-Quinones）或黄酮的结构活性关系。最活跃的化合物是奎因酮中的拉帕醇（3-羟基-4-丙二芬酮），在黄酮中未取代的黄酮或未取代的黄酮或5-甲氧基氟酮。 3。抗MBC的抗毒剂的作用方式，该剂被认为是微管蛋白聚合的抑制剂，因此导致有丝分裂的衰竭非常感兴趣。据我们目前所知，上面提到的解毒剂在体外没有与MBC竞争β-微管蛋白的结合位点。 4。分别从piscidia erythrina（豆科）分离并阐明了一些生理活性的次级代谢产物，异黄素，倍半萜衍生物和寡素衍生物； Rosa Rugosa（酒渣鼻）和Japonicus藻类和C. serratus（氯菊科）；和Carex spp。（Cypreaceae）。