Improvement of Silica Gel Column Packing Materials - pursuit of Functionality -

硅胶柱填料的改进-追求功能性-

• 批准号: 02453145
• 负责人: AKIYAMA Shuzo
• 金额: $ 4.16万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02453145/
• 关键词: silica gel; silica gel column packing material; high-performance liquid chromatography; 3-aminopropylsilyl silica gel; nucleic acid related substance; water-soluble vitamins; chemically modified gel; (3ーモルホリノプロピルシ)シリルシリカゲル; 化学修飾; 健康ドリンク剤

To begin with, three types of high-performance liquid chromatographic column packing materials ((3-morpholinopropyl)-[3-(1-pirazynyl) propyl]-, and (3-piperidinopropyl) silyl silica gels) were prepared and examined for their ability to separate water-soluble organic compounds such as water-soluble vitamins. Six vitamins (ascorbic acid, nicotinic acid, thiamine hydrochloride, pyridoxine hydrochloride, hydroxocobalamin acetate, and flavin adenine dinucleotide) in sample solutions could be completely separated within less than 12 min by UV detection on a column packed with morpholinopropylsilyl silica gel (MPS) by using a phosphate buffer as an eluent. The MPS column was successfully used also for the determination of three nucleotidic substances (5'-inosinic acid, 5'-guanylic acid and 5'-xanthylic acid) in seasonings and the mixture of adenosine morno-, di- and triphosphates (AMP, ADP and ATP). Next, naphthoyl- and anthroylaminopropylsilyl silica gels were newly prepared by the reaction of 3-aminopropylsilyl silica gel with the corresponding acid chlorides and found to be suitable for the separation of the nucleic acid related compounds above mentioned including the related nucleosides. The results are now being applied to the measurement of an ATPase activity. Patents on the preparation and the performance of MPS and the related gels was successfully applied.

首先，制备并检查了三种类型的高效液相色谱柱填料材料（（（3-多酚丙基） - [3-（1-Pirazynyl）丙基] - 和（3-二哌迪丙基）的硅胶凝胶）进行了检查并检查了它们的能力，以便它们能够分离出水溶性有机化的有机化合物等能力。六种维生素（抗坏血酸，烟酸，硫胺素盐酸盐，吡啶还醇盐氯化物，乙酸羟基盐酸盐剂，乙酸羟基盐和黄素腺嘌呤二核苷酸）在样品溶液中可以通过在较小的12分钟内通过在少于12分钟内通过甲填充（通过圆柱）在紫外线的速圈（Mets Aneprate ands Ane）内完全分离出来。洗手。 MPS柱也成功地用于测定调味剂中的三种核苷酸物质（5'-肌酸，5'-鸟舍酸和5'- Xanthylic Acid），以及腺苷MORNO-，二磷酸盐和三磷酸腺苷（AMP，ADP和ATP）的混合物。接下来，通过3-氨基丙基硅烷基硅烷基硅烷二氧化硅凝胶与相应的酸性氯化物的反应新制备了萘甲酰基和氨基氨基丙基二硅烷基硅烷基硅烷基硅烷基硅烷基硅烷基凝胶，并发现适合上述相关化合物的分离上述化合物，包括相关化合物，包括相关核苷。现在，结果应用于ATPase活性的测量。成功应用了MPS和相关凝胶的制备和性能的专利。

项目成果

Nakashima,Kenichiro: "Peroxyoxalate Chemiluminescence Assay of Hydrogen Peroxide and Glucose Using 2,4,6,8-Tetrathiomorpholino-pyrimido〔5,4,-d〕pyrimidine as a Fluorescent Component" Anal.Sci.7. 709-713 (1991)

Nakashima, Kenichiro：“使用 2,4,6,8-四硫代吗啉代-嘧啶并[5,4,-d]嘧啶作为荧光成分对过氧化氢和葡萄糖进行过氧草酸盐化学发光测定”Anal.Sci.7 (1991)。 ）

Nakashima,Kenichiro: "High Performance Liquid Chromatography with Chemilumines-cence Detection of Methamphetamine and Amphetamine in Human Urine" Anal.Sci.7. 815-816 (1991)

Nakashima，Kenichiro：“用化学发光法检测人尿液中的甲基苯丙胺和苯丙胺的高效液相色谱法”Anal.Sci.7。

K. Nakashima: "High-Performance Liquid Chromatography-Chemiluminescence Determination of Methamphetamine in Human Serum" J. Chromatogr.530.

K. Nakashima：“高效液相色谱-化学发光测定人血清中的甲基苯丙胺”J. Chromatogr.530。

Akiyama,Shuzo: "HighーPerformance Liquid Chromatographic Determination of Components in Eye Lotion Using Methoxy(3ーmorpholinoーpropyl)silanediyl modified Silica Gel Column" Bull.Chem.Soc.Jpn.64. 3171-3172 (1991)

Akiyama, Shuzo：“使用甲氧基（3-吗啉代-丙基）硅烷二基改性硅胶柱高效液相色谱测定眼部乳液中的成分”Bull.Chem.Soc.Jpn.64（1991）。

Nakashima,Kenichiro: "High Performance Liquid Chromatography with Chemiluminesーcence Detection of Methanphetamine and Amphetamine in Human Urine" Anal.Sci.7. 815-816 (1991)

Nakashima, Kenichiro：“用化学发光法检测人尿液中的甲基苯丙胺和安非他明的高效液相色谱法”Anal.Sci.7 (1991)。