Physiological and Morphological Studies on Characteristics of Nociceptive Primary Afferent Neurons (Polymodal Receptors).

伤害性初级传入神经元（多模式受体）特征的生理学和形态学研究。

• 批准号: 01480122
• 负责人: KUMAZAWA Takao
• 金额: $ 3.84万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480122/
• 关键词: Pain; Nociceptor; Inflammation; Intraspinal termination; Neurogenic inflammation; Neuropeptide; Inflammatory mediator; 脊髄内終末; 脊髄内終未

1. In order to clarify changes in polymodal receptor activities in inflammation, effects of inflammatory mediators on the heat response of testicular polymodal receptors were studied in vitro using testis-spermatic nerve preparations. ProstaglandinE_2 augmented the heat responses from the concentration 100 times higher than that needed to augment the Bradykinin (BK) response. 5-HT augmented both responses in the same range of concentration. Histamine (His) had different effects : His suppressed or augmented the BK response depending on whether His induced substantial increase in discharge rate or not, whereas it always augmented the heat response. BK augmented the heat response. None of these mediators could augment the heat response so strong as 55^ﾟC-30s heat stimulation did. BK response was revealed to be mediated by B2 type receptor. 2. Peripheral structure of identified testicular polymodal receptors was clarified by EM. Spinal termination of identified testicular polymodal receptors was traced mainly in lamina I by intracellular injection of PHA-L. Double labeling with fluorescent dyes revealed that more than 90% DRG neurons innervating the testis contained CGRP. 3. Nervous component in changes in intraocular pressure induced by inflammatory mediators was clarified, but the possibility for substance P to be its neuronal mediator was not supported.

1。为了阐明炎症中多峰受体活性的变化，使用睾丸 - 乳清神经神经制剂在体外研究了炎症介质对睾丸多型受体热反应的影响。 Prostaglandine_2增强了浓度的热反应比增强心动激肽（BK）反应所需的热反应。 5-HT在相同浓度范围内增强了这两个反应。组胺（他的）产生不同的影响：根据他是否诱导出院率的大幅度增加，他的抑制或增强了BK反应，而它始终增加了热响应。 BK增加了热响应。这些介体都无法增加像55^C-30S热刺激的热反应。 BK反应显示是由B2型受体介导的。 2。通过EM阐明已鉴定的测定多峰受体的外周结构。通过细胞内注射PHA-L，主要在椎板I中追溯了鉴定出的多峰受体的脊柱终止。用荧光染料的双重标记表明，支配睾丸的90％以上的DRG神经元包含CGRP。 3。阐明了炎症介质引起的眼内压变化的神经成分，但不支持物质P为其神经元介质的可能性。

项目成果

Mizumura,K.et al.: "Comparison of effects of prostaglandinE_2 and I_2 on testicular nociceptor activities studied in vitro" NaunynーSchmiedeberg's Arch.Pharmacol.

Mizumura, K. 等人：“体外研究的前列腺素 E_2 和 I_2 对睾丸伤害感受器活性的影响比较”Naunyn-Schmiedeberg 的 Arch.Pharmacol。

Mizumura, K. et al.,: "Comparison of effects of prostaglandinE_2 and I_2 on testicular nociceptor activities studied in vitro" Naunyn-Schmiedeberg's Arch. Pharmacol.,.

Mizumura, K. 等人：“体外研究的前列腺素 E_2 和 I_2 对睾丸伤害感受器活性的影响比较”Naunyn-Schmiedebergs Arch。

K.Mizumura: "Reversible suppressive effects of amfenac sodium on the response to bradykinin of the visceral polymodal receptor." Environmental Medicine. 33. 43-46 (1989)

K.Mizumura：“氨芬酸钠对内脏多模式受体缓激肽反应的可逆抑制作用。”

K.Yokoyama: "Implication of polymodal receptor activities in the intraocular pressure increase resulted from neurogenic inflammation." Japanese Journal of Ophthalmology. 34. (1990)

K.Yokoyama：“多模式受体活性对神经源性炎症引起的眼内压升高的影响。”

Mizumura,K.et al.: "proceedings of the VIth Congress on Pained.by Bond,M.R.et al." Elsevier(Amsterdam),

Mizumura,K.et al.：“第六届 Pained 大会记录，作者：Bond,M.R.et al.”