Studies on plasma membrane proteins which are specifically induced by cold acclimation in plants.

植物冷驯化特异性诱导的质膜蛋白的研究。

基本信息

批准号：
01480009
负责人：
YOSHIDA Shizuo
金额：
$ 3.78万
依托单位：
(The Institute of Low Temparature Science) Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

The objective of this study was to identify plasma membrane proteins which are specifically induced by cold acclimation in wheats (Triticum aestivum L.). Two cultivars with a marked difference in the genetic capability to harden, namely spring wheat13EA\ : (cv Chinese Spring) and winter wheat (cv Norstar) were used as the experimental materials. After four weeks of growth in a cold chamber, the freezing tolerance in the shoots of winter wheat increased to -18ﾟC, whereas only to -8ﾟC in the shoots of spring wheat. In roots from both cultivars, the freezing tolerance increased very slightly after the growth under the cold environment. Cold hardening induced remarkable changes in electrophoresis patterns of plasma membrane proteins depending on c13EA\ : ultivars and tissues. Polypeptides with molecular masses ranged from 22 to 31 kDa decreased commonly in the root and shoot plasma membranes from both cultivars. of these polypeptides, 28 and 26 kDa polypeptides decreased abrupty after one week of th13EA\ : e cold hardening. On the other hand, polypeptides with molecular masses of 89, 83, 23, 18 and 17 kDa increased specifically in the shoots of winter hardy wheat. The increases of the 23, 18 and 17 kDa polypeptides were proportional to the development13EA\ : of freezing tolerance. A freeze-fracture electronmicroscopy of plasma membranes from shoot cells revealed that the intramembrane particle number on the fracture faces decreased significantly in winter wheat after cold acclimation, but less in sprin13EA\ : g wheat. These results may suggest that the plasma membrane undergoes a molecular reorganization during cold acclimation in such manner that they are stabilized against freeze-induced dehydration stress.

本研究的目的是鉴定小麦（Triticum aestivum L.）中冷驯化特异性诱导的质膜蛋白，这两个品种在遗传硬化能力方面存在显着差异，即春小麦13EA\：（cv Chinese Spring）。以冬小麦(cv Norstar)为试验材料，在冷室中生长4周后，冬小麦芽的耐冻性提高到-18℃，而冬小麦芽的耐冻性仅提高到-18℃。 -8°C 下，两个品种的根部在寒冷环境下生长后，其抗冻性均略有增加，这导致质膜蛋白的电泳模式发生显着变化，具体取决于 c13EA\ ：品种和。分子量范围为 22 至 31 kDa 的多肽在这两个品种（28 和 26 kDa 多肽）的根和芽质膜中普遍降低。 th13EA\：e 冷硬化一周后突然增加，另一方面，多肽的分子量为 89、83、23、18 和 17 kDa，特别是在耐寒小麦的芽中增加。 13EA1和17kDa多肽与冷冻耐受性的发展成正比。来自芽细胞的质膜的冷冻断裂电子显微镜显示，冷驯化后，冬小麦断裂面上的膜内颗粒数量显着减少，但 sprin13EA\ : g 小麦的膜内颗粒数量减少。这些结果可能表明质膜在冷驯化过程中经历了分子重组，从而使其能够稳定地抵抗冷冻。诱发脱水应激。