Studies on plasma membrane proteins which are specifically induced by cold acclimation in plants.

植物冷驯化特异性诱导的质膜蛋白的研究。

基本信息

批准号：
01480009
负责人：
YOSHIDA Shizuo
金额：
$ 3.78万
依托单位：
(The Institute of Low Temparature Science) Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

The objective of this study was to identify plasma membrane proteins which are specifically induced by cold acclimation in wheats (Triticum aestivum L.). Two cultivars with a marked difference in the genetic capability to harden, namely spring wheat13EA\ : (cv Chinese Spring) and winter wheat (cv Norstar) were used as the experimental materials. After four weeks of growth in a cold chamber, the freezing tolerance in the shoots of winter wheat increased to -18ﾟC, whereas only to -8ﾟC in the shoots of spring wheat. In roots from both cultivars, the freezing tolerance increased very slightly after the growth under the cold environment. Cold hardening induced remarkable changes in electrophoresis patterns of plasma membrane proteins depending on c13EA\ : ultivars and tissues. Polypeptides with molecular masses ranged from 22 to 31 kDa decreased commonly in the root and shoot plasma membranes from both cultivars. of these polypeptides, 28 and 26 kDa polypeptides decreased abrupty after one week of th13EA\ : e cold hardening. On the other hand, polypeptides with molecular masses of 89, 83, 23, 18 and 17 kDa increased specifically in the shoots of winter hardy wheat. The increases of the 23, 18 and 17 kDa polypeptides were proportional to the development13EA\ : of freezing tolerance. A freeze-fracture electronmicroscopy of plasma membranes from shoot cells revealed that the intramembrane particle number on the fracture faces decreased significantly in winter wheat after cold acclimation, but less in sprin13EA\ : g wheat. These results may suggest that the plasma membrane undergoes a molecular reorganization during cold acclimation in such manner that they are stabilized against freeze-induced dehydration stress.

这项研究的目的是鉴定质膜蛋白是由小麦（Triticum aestivum L.）中冷适应的特异性诱导的。两个品种在硬化的遗传能力上有明显差异，即春季小麦13ea \：（CV中国春季）和冬小麦（CV Norstar）用作实验材料。经过四个星期的冷室生长，冬小麦芽的冻结耐受性增加到-18°C，而春季小麦的芽中仅为-8 c。在两个品种的根中，在寒冷环境下生长后，冻结的耐受性略有略微增加。冷硬化引起的质膜蛋白电泳模式的显着变化取决于C13EA \：Ultivars和Tisues。分子质量的多肽范围为22至31 kDa在根中通常降低，并且来自两个品种的质质膜。在这些多肽中，有28和26 kDa多肽在13ea \：e冷硬化后的一周后降低。另一方面，分子质量为89、83、23、18和17 kDa的多肽特别在冬季硬小麦的芽中增加了。 23、18和17 kDa多肽的增加与发育13EA \：冻结耐受性成正比。芽细胞中质膜的冻结电子显微镜表明，骨折面部的膜内颗粒数在冷适应后的冬小麦中显着降低，但在Sprin13ea \：G小麦中较少。这些结果可能表明，质膜在冷适应过程中经历了分子重组，以使它们稳定在冻结诱导的脱水应力上。