Functional characterization of Fe-dependent mechanisms promoting the stimulation of adventitious root formation in petunia cuttings

促进矮牵牛插条不定根形成的铁依赖性机制的功能表征

• 批准号: 424125725
• 负责人: Dr. Mohammad-Reza Hajirezaei
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/424125725?language=en
• 关键词: Functional; characterization; Fe; dependent; mechanisms

Adventitious root (AR) formation is prerequisite for vegetative propagation of important crops and common practice to maintain the genetic identity among progenies. With increasing use of soil-free media in high-throughput propagation and growing demand for sustainable horticultural practices, a deep understanding of the role of mineral nutrition in AR formation is essential for the improvement of existing propagation protocols. Our previous work demonstrated that Fe is the most limiting nutrient for AR formation and provided compelling evidence for a unique unexploited role of Fe in stimulation of meristematic cell division, associated with its high accumulation in the nuclei of meristematic cells. The proposed project aims to study the physiological and biochemical mechanisms underlying Fe-stimulated AR formation and to identify genes that can be used to improve AR formation. Initially, a detailed cell biological analysis and ultrastructural localization of Fe will define the major anatomical and physiological processes upon Fe application. A comprehensive transcriptome profiling using RNAseq and MS-based analysis of nucleolar proteins will identify genes and proteins involved in Fe-accelerated AR formation. The function of top candidate genes derived from proteomic and transcriptomic studies will be characterized in transposon-insertion or CRISPR/Cas9-modified transgenic plants using anatomical and physiological approaches. Finally, the Fe-dependent AR formation will be studied in wild petunia accession lines, closely related Solanaceae genera such as tomato gene pool for a better access to additional mutants and several commercial cultivars of horticultural crops. The proposed study will advance the understanding of the unique function of Fe in the plant nucleolus and will pave the ground for a targeted exploitation of Fe nutrition in vegetative propagated crops

不定根（AR）的形成是重要农作物的营养传播和普通实践的前提，以维持后代之间的遗传认同。随着无土培养基在高通量传播中的使用越来越多，对可持续园艺实践的需求不断增长，对矿物营养在AR形成中的作用的深入了解对于改善现有传播方案至关重要。我们以前的工作表明，FE是AR形成的最有限的养分，并为FE在刺激分生组织细胞分裂中发挥了独特的未开发作用提供了令人信服的证据，这与其在分生细胞细胞核中的高积累有关。拟议的项目旨在研究Fe刺激的AR形成的生理和生化机制，并鉴定可用于改善AR形成的基因。最初，FE的详细细胞生物学分析和超微结构定位将定义Fe应用后的主要解剖和生理过程。使用RNASEQ和基于MS的核仁蛋白分析的综合转录组分析将鉴定参与Fe加速AR形成的基因和蛋白质。源自蛋白质组学和转录组研究的顶级候选基因的功能将以解剖学和生理方法的形式在转座子插入或CRISPR/CAS9修饰的转基因植物中进行表征。最后，将在野生的矮牵牛登录系中研究依赖性的AR形成，这是密切相关的茄科属，例如番茄基因库，以更好地获取其他突变体和几种园艺作物的商业品种。拟议的研究将提高对植物核仁中铁的独特功能的理解，并将为靶向剥削Fe营养而在营养繁殖的农作物中奠定基础。