Bright field, phase contrast and epifluorescence microscopy for characterizing phenotypes of transgenic isolates of sclerotinia species

明场、相差和落射荧光显微镜用于表征菌核菌转基因分离株的表型

• 批准号: 344655-2007
• 负责人: Boland, Gregory
• 金额: $ 1.79万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325561
• 关键词: Bright; field; phase; contrast; epifluorescence

Plant health is an important component of food, fibre and environmental sustainability in Canada and elsewhere.  Plant diseases are an important component of plant health and result in significant losses in agriculture, horticulture and forestry every year. Most plant pathogens are microorganisms and, therefore, a microscope is considered a core and critical piece of equipment in a plant pathology laboratory.   The microscope proposed in this application will be used in several research projects focused on the understanding and development of biological controls for plant diseases.  The influence and mechanisms of action of fungal viruses and how they influence their fungal hosts must be characterized to enable their development as effective procedures for disease management.  Selected fungal viruses associated with reduced virulence and debilitated growth in fungal plant pathogens have been identified in previous studies, and current studies are focused on determining how these viruses affect their fungal hosts.  Genetic transformation of fungal hosts with selected fragments of the viral genome requires a research-quality microscope for detection of the influence of these changes on the growth and development of these microscopic fungi.   This proposal will replace an existing and out-dated light microscope with a comparable unit that has an enhanced feature for epi-fluorescence illumination and digital photography.  Importantly, the digital photography system with this microscope is compatible with other equipment in the laboratory.   This microscope will enable highly qualified personnel (HQP) to continue currently-funded and on-going research and diagnostic activities, and expand into research areas that require more precise and technical application, such as observing fluorescent dyes and proteins (e.g. epi-fluorescence) in fungal and plant tissues during growth and infection.

植物健康是加拿大和其他地区食品、纤维和环境可持续性的重要组成部分。植物病害是植物健康的重要组成部分，每年都会给农业、园艺和林业造成重大损失，因此，大多数植物病原体都是微生物。显微镜被认为是植物病理学实验室的核心和关键设备。​本申请中提出的显微镜将用于多个研究项目，重点是了解和开发疾病的生物控制作用。的必须确定真菌病毒及其如何影响真菌宿主的特征，以使其发展成为疾病管理的有效程序，之前的研究已确定与真菌植物病原体毒力降低和生长衰弱相关的选定真菌病毒，目前的研究重点是。确定这些病毒如何影响其真菌宿主，用选定的病毒基因组片段对真菌宿主进行遗传转化需要研究级显微镜来检测这些变化对这些微生物生长和发育的影响。该提案将用具有增强的落射荧光照明和数字摄影功能的类似装置取代现有的过时的光学显微镜。重要的是，该显微镜的数字摄影系统与实验室的其他设备兼容。该显微镜将使高素质人员 (HQP) 能够继续当前资助和正在进行的研究和诊断活动，并扩展到需要更精确和技术应用的研究领域，例如观察荧光染料和蛋白质（例如荧光染料和蛋白质）。生长和感染期间真菌和植物组织中的落射荧光）。