Point-of-care therapeutic monitoring of anti-bacterial using low-cost disposable sensor

使用低成本一次性传感器对抗菌进行护理点治疗监测

• 批准号: 22K18187
• 负责人: Aaryashree
• 金额: $ 2.83万
• 依托单位: Shibaura Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K18187/
• 关键词: vancomycin; MIP carbon paste; TDM; MIP; disposable sensor

Monitoring the levels of antimicrobial drugs is crucial to avoid toxic effects. Therapeutic drug monitoring (TDM) is a helpful tool to achieve this. We have developed a vancomycin (VCM) sensor to monitor drug concentration in whole blood. However, current sensor stability and VCM sensitivity depend on chip base structure and amperometric measurement mode. Previously, we had two major problems: the molecularly imprinted polymer-grafted-carbon paste (MIP-CP) was not reproducible, and the chip sensor lost sensitivity within three days of packing. After several optimizations, we have successfully made a highly reproducible MIP-CP. This chip has good batch reproducibility and good long-term stability. Also, the long-term stability of the packed chip has improved to two weeks in buffer saline and ten days in whole bovine blood. This indicates a good improvement over the past chip sensor. The data has been successfully disseminated through three conferences.

监测抗菌药物的水平对于避免有毒作用至关重要。治疗药物监测（TDM）是实现此目标的有用工具。我们已经开发了万古霉素（VCM）传感器来监测全血的药物浓度。但是，当前的传感器稳定性和VCM敏感性取决于芯片底座结构和安培测量模式。以前，我们遇到了两个主要问题：分子烙印的聚合物夹碳糊（MIP-CP）不可再现，并且芯片传感器在包装后的三天内失去了灵敏度。经过几次优化后，我们成功地制造了高度可重现的MIP-CP。该芯片具有良好的批处理可重复性和良好的长期稳定性。同样，包装芯片的长期稳定性在缓冲盐水中提高到两周，在整个牛血中十天。这表明对过去的芯片传感器有很好的改进。数据通过三个会议成功传播。

项目成果

A Disposable Chip Sensor for the Detection of Vancomycin: Studying the Long-Term Stability

用于检测万古霉素的一次性芯片传感器：研究长期稳定性

• 发表时间: 2022
• 作者: Aaryashree,Ashish Kumar Choudhary;Akihiko Hatano and Yasuo Yoshimi
• 通讯作者: Akihiko Hatano and Yasuo Yoshimi

Development of antimicrobial drug chips using molecularly imprinted polymer grafted-carbon paste on screen-printed electrode

丝网印刷电极上分子印迹聚合物接枝碳糊开发抗菌药物芯片

• 发表时间: 2022
• 作者: Hiarta Aoi;Yasuo Yoshimi;Aaryashree
• 通讯作者: Aaryashree

Realtime Detection of Vancomycin in Human Whole Blood using Disposable Sensor Chip Electrode

使用一次性传感器芯片电极实时检测人全血中的万古霉素

• 发表时间: 2022
• 作者: Aaryashree;Choudhary Ashish;Yasuo Yoshimi;Akihiko Hatano;Ken-ichi Saito
• 通讯作者: Ken-ichi Saito