Creation and biological evaluation of nanoparticle-based artificial transcription factor

基于纳米颗粒的人工转录因子的创建和生物学评价

基本信息

批准号：
22K15249
负责人：
CHINNATHAMBI Shanmugavel
金额：
$ 3万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Early-Career Scientists
财政年份：
2022
资助国家：
日本
起止时间：
2022-04-01 至 2024-03-31
项目状态：
已结题

项目摘要

Silicon quantum dots (SiQDs) capable of in vitro and in vivo imaging could dissect cellular function at the molecular level and have been advancing as a potent diagnostic tool in precision medicine. While numerous reports focus on evaluating the staining capacity of silica quantum dots, their nano-bio interaction, which is critical for assessing their biocompatibility, is often overlooked. We developed water-borne, NIR-emitting Pluronic F127-covered SiQDs micelles capable of live cell imaging with minimal cytotoxicity. The PLQY of the SiQDs/F127 micelles in water was 25% at pH 7. SiQD-De/F127 micelles demonstrated significant in vitro imaging capacity and limited cytotoxicity in the HeLa and human dermal fibroblasts. Furthermore, the SiQD-De/F127 micelles displayed excellent in vivo imaging capacity without requiring vectors and transfection agents when incubated with medaka fish eggs and larvae. It is important to note here that even at higher concentrations (1 mg mL－1), the normal embryonic development of medaka fish was not altered as the larvae hatched on day 17 from the micelles-treated egg as that of the control without any signs of toxicity. Therefore, SiQDs-De/F127 micelles are SiQDs expected to have excellent biocompatibility for in vivo imaging studies.

能够进行体外和体内成像的硅量子点 (SiQD) 可以在分子水平上剖析细胞功能，并已成为精准医学领域的有效诊断工具。纳米生物相互作用对于评估其生物相容性至关重要，但常常被忽视。我们开发了水性、近红外发射的 Pluronic F127 覆盖的 SiQD 胶束，能够以最小的速度进行活细胞成像。 SiQDs/F127 胶束在 pH 7 时在水中的 PLQY 为 25%。SiQD-De/F127 胶束在 HeLa 和人真皮成纤维细胞中表现出显着的体外成像能力和有限的细胞毒性。当与青鳉鱼卵一起孵育时，胶束表现出优异的体内成像能力，无需载体和转染剂这里值得注意的是，即使在较高浓度（1 mg mL−1）下，青鳉鱼的正常胚胎发育也没有改变，因为幼虫在第 17 天从经过胶束处理的卵中孵化出来，与未经胶束处理的对照卵一样。因此，SiQDs-De/F127 胶束预计对体内成像研究具有优异的生物相容性。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A biophysical approach of cytarabine anticancer drug insights into human serum albumin and checkpoint kinase 1

阿糖胞苷抗癌药物的生物物理方法深入了解人血清白蛋白和检查点激酶 1

DOI：
10.1016/j.rechem.2022.100755
发表时间：
2023
期刊：
Results in Chemistry
影响因子：
2.3
作者：
Rupavarshini Manoharan;Karthikeyan Subramani;Anandh Sundaramoorthy;Ramamoorthi Anitha;Ramakrishnamurthy Suganya;Bharanidharan Ganesan;Aruna Prakasarao;Mangaiyarkarasi Rajendiran;Chinnathambi Shanmugavel;Pandian Ganesh N.;Ganesan Singaravelu
通讯作者：
Ganesan Singaravelu

Vellore Institute of Technology, Chennai(インド)

韦洛尔理工学院，金奈（印度）

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Facile Synthesis of Hafnium Oxide Nanoparticle Decorated on Graphene Nanosheet and Its Photocatalytic Degradation of Organic Pollutants under UV-Light Irradiation

石墨烯纳米片修饰氧化铪纳米粒子的简便合成及其在紫外光照射下光催化降解有机污染物

DOI：
10.3390/app122111222
发表时间：
2022
期刊：
Applied Sciences
影响因子：
0
作者：
Jayaraman Venkatachalam;Mahalingam Shanmugam;Chinnathambi Shanmugavel;Pandian Ganesh N.;Prakasarao Aruna;Ganesan Singaravelu;Ramasamy Jayavel;Ayyaru Sivasankaran;Ahn Young-Ho
通讯作者：
Ahn Young-Ho

University of Science(マレーシア)

理科大学（马来西亚）