Differential dopamine dynamics of DMS and DLS projecting SNc neurons during reversal learning

逆转学习过程中 DMS 和 DLS 投射 SNc 神经元的差异多巴胺动力学

• 批准号: 21K15184
• 负责人: RIOS・DAVILA ALAIN・ANTONIO
• 金额: $ 3万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K15184/
• 关键词: Dopamine; Basal Ganglia; Goal-directed behavior; dLight; Striatum; Basal ganglia; Goal directed behavior

Elucidation of the changes of the phasic activity of the DMS and DLS projecting SNc neurons, identified direct and indirect pathway striatal neurons, and local striatum dopamine release in rats performing a probabilistic reward-based task. We used TH-Cre, Drd2-Cre and Tac-1-Cre transgenic rats for dopaminergic neurons and striatal projecting neurons identification. For the investigation of dopamine release in dorsal striatum, we express dLight in DMS and DLS and monitored the fluorescence using fiber photometry.Elucidation of the differential role of intratelencephalic (IT) and corticofugal (CF) DMS corticostriatal OFC and mPFC in rats performing a probabilistic reward-based task. We use a viral vector approach for the identification of the different DMS corticostriatal neurons.

阐明了DMS和DLS投射SNC神经元的变化，确定了直接和间接途径纹状体神经元，以及在执行基于概率奖励任务的大鼠中局部纹状体多巴胺释放。我们将TH-CRE，DRD2-CRE和TAC-1-CRE转基因大鼠用于多巴胺能神经元和纹状体突出神经元鉴定。为了研究多巴胺在背纹状体中的释放，我们在DMS和DLS中表达DLIMPLE，并使用纤维光度法来监测荧光。溶解了脑脑内（IT）和皮质表征（CF）DMS Corticososticostriatal ofc和MPFC的差异作用，以造成大衣的基于大衣的奖励奖励。我们使用病毒载体方法来鉴定不同的DMS皮质纹状体神经元。

项目成果

Dynamic and pathway-specific signaling in the dorsomedial striatum and dopamine neurons during behavioral adaptation

行为适应过程中背内侧纹状体和多巴胺神经元的动态和通路特异性信号传导

• 发表时间: 2022
• 作者: Rios Alain