連続脳切片を用いた神経突起の三次元再構築のためのステ-ジ連動型描画装置の試作

使用连续脑切片三维重建神经突的阶段联动绘图装置原型

• 批准号: 63870008
• 负责人: SHINODA Yoshikazu
• 金额: $ 4.16万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63870008/
• 关键词: Reconstruction device; Neuron; Microscope; Golgi staining; HRP staining; 形態解析; 三次元再構築; 三次元コンピュ-タ-解析描画装置; 三次元コンピュ-ター解析描画装置; HRP; 前庭核; 脊髄; 小脳; 細胞内染色

To accelerate the speed of analysis on three dimensional structures of single neurons, a new reconstruction device was developed. This device was composed of a conventional microscope with a camera lucida drawing attachment, a motor-driven microscope stage and a motor-driven table which can be moved in X Y directions. The motor-driven tables moves in proportion to the movement of the motor-driven stage by a microprocessor system, so that the desired structures on the brain slice under a microscope can be drawn through the drawing attachment and then, by moving the neighboring structures just outside the view of the microscope in to the viewer, those structures can be described continuously. By repeating this procedure, the whole structure of a single neuron on one slice can be traced. Serial sections are processed in the same way. Finally, each drawing of one brain section adjusted one by one to connect cut ends of neuronal structures of one slice to the appropriate cut ends on the previous slice. This device can trace a neuronal structure and enlarge it to 1.5m wide x 2 m long. The reconstruction time is speeded by three times.

为了加快单个神经元三维结构的分析速度，开发了一种新的重建装置。该设备由带有相机Lucida绘图附件的传统显微镜组成，电动机驱动的显微镜阶段和一个可以沿x y方向移动的电动驱动台。电机驱动的桌子与微处理器系统的运动驱动阶段的运动成比例，因此可以通过图形附件绘制显微镜下的大脑切片上的所需结构，然后通过将相邻的结构移动到视图中的显微镜视图之外，可以持续描述这些结构。通过重复此过程，可以追溯到一个切片上单个神经元的整个结构。串行部分以相同的方式处理。最后，一个大脑部分的每条图都逐一调整，以将一个切片的神经元结构的切割末端连接到上一个切片上适当的切割末端。该设备可以追踪神经元结构，并将其扩大到1.5m宽的x 2 m长。重建时间加快了三次。

项目成果

Shinoda,Y.,S.Kakei: "Distribution of terminals of thalamocortical fibers originating from the ventrolateral nucleus of the cat thalamus" Neuroscience Letters. 96. 163-167 (1989)

Shinoda，Y.，S.Kakei：“源自猫丘脑腹外侧核的丘脑皮质纤维末端的分布”神经科学快报。

Shinoda, Y.: "Vestibular projections to the spinal cord. In "Vestibular control of posture and movement."" Prog. in Brain Res. Vol.76 (17-27) 1988.

Shinoda, Y.：“前庭对脊髓的投射。在“姿势和运动的前庭控制”中。”Prog。

Shinoda,Y.et al.: "Spatial innervation patterns of single vestibulospinal axons in neck motor nuclei" Head-Neck Sensory-Motor System Berthoz,A.(Ed),IBRO Symposium. (1990)

Shinoda,Y.et al.：“颈部运动核中单个前庭脊髓轴突的空间神经支配模式”头颈感觉运动系统 Berthoz,A.（编辑），IBRO 研讨会。

篠田義一: "新生理学大系8神経生理学総論破壊と刺激" 伊藤正男,塚原仲晃, 132-150 (1989)

筱田义一：《新生理学系统8神经生理学通论破坏与刺激》Masao Ito，Nakaaki Tsukahara，132-150（1989）

Shinoda,Y.: Progress in Brain Research. 76. 17-27 (1988)

Shinoda，Y.：大脑研究进展。