Development of a Specific Micro-determination Method of Gizzerosine, an Inducer of Gizzard Erosion in Chicken

鸡砂囊糜烂诱导剂 Gizzerosine 的特异性微量测定方法的建立

• 批准号: 60860009
• 负责人: NAITO Hiroshi
• 金额: $ 5.7万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60860009/
• 关键词: gizzerosine; histamine; high-performance liquid chromatography; gizzard erosion; histamine receptor; fish meal; ジゼロシン; ヒスタミン; モノクローナル坑体; 筋胃潰瘍; 魚粉; 胃酸分泌リジン; アスコルビン酸

1. A fluorometric determination method of gizzerosine was developed employing high-performance liquid chromatography. By this method, 0.2 to 5.0 g of gizzerosine in Fish meals was quantitated with the recovery of 95-102 %. fish meals, which induced severe gizzard erosion in chicks, contained more than 20 mg/kg of gizzerosine.2. Employing the newly developed determination method, the conditions of gizzerosine formation in fish meals were examined. When the pH of fish meat was slightly acidic and when the temperature during drying was more than 100゜C, significant amount of gizzerosine was formed in the fish meal.3. If lysine or ascorbic acid was included in fish meat before drying, the formation of gizzerosine was prevented. This method made it possible to produce low-gizzerosine fish meal.4. Mucosal cells of chicken proventriculus were prepared employing collagenase digestion method. Gizzerosione enhanced the oxygen uptake by these cells and the activity was inhibited by antagonists of histamine H_2-receptor. This suggests that gizzard erosion induced by gizzerosine can be prevented by cimetidine and its related compounds. This is anothor possible method to prevent gizzard erosion in chicks.5. The above-mentioned determination method was relatively complex. So, another method was tried to be developed. By using ion-exchange high performance liquid chromatography, gizzerosine was determined within 1 or 2 days. A practical procedure for wide usage of this method is now being developed.

1。鸡在95-102％的回收率中进行了定量。干燥大于大于100°C，在鱼粉中形成了大量的gizzerosine。3。通过Cimetide及其相关的化合物，这是鸡的另一种可能的方法。天。

项目成果

Yoshinori Ito,; Tadashi Noguchi; Hhiroshi Naito: "Fluorometric Determination of Gizzerosine, a Histamine H_2-Receptor Agonist Discovered in Feedstuffs, Employing High-Performance Liquid Chromatography." Analytical Biochemistry. 151. 28-31 (1985)

伊藤嘉德;

Tsuneo Hino,;Tadashi Noguchi,;Hiroshi Naito: Poultry Science. 66. 548-551 (1987)

Tsuneo Hino，；Tadashi Noguchi，；Hiroshi Naito：家禽科学。

Hiroshi Naito,;Yoshinori Ito,;Tadashi Noguchi: "Amino-Carbonyl Reaction in Food and Biological Systems---Proceedings of the 3rd International Symposium on the Maillard Reaction" Kodansha,Ltd.Elsevier, 588 (1986)

Hiroshi Naito,;Yoshinori Ito,;Tadashi Noguchi：“食品和生物系统中的氨基-羰基反应---第三届美拉德反应国际研讨会论文集”讲谈社，Elsevier，588（1986）

ANALYTICAL BIOCHEMISTRY. 151-1. (1985)

分析生物化学。

Hiroshi Naito,Yoshinori Ito and Tadashi Noguchi: "Amino-Carbonyl Reaction in Food and Biological Systems---Proceedings of the 3rd International Symposium on the Maillard Reaction" Kodansha,Ltd.Tokyo Elserier Amsterdam, 588 (1986)

Hiroshi Naito、Yoshinori Ito 和 Tadashi Noguchi：“食品和生物系统中的氨基-羰基反应 --- 第三届美拉德反应国际研讨会论文集”讲谈社，有限公司东京 Elserier 阿姆斯特丹，588 (1986)