Studies on Technique for Preparation and Isolation of a Large Amount of Oligosaccharides and on Utilization of Oligosaccharides.

大量低聚糖的制备、分离技术及低聚糖的利用研究。

基本信息

批准号：
59860008
负责人：
SEIYA Chiba
金额：
$ 2.62万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59860008/
关键词：
Oligosaccharide <alpha> -Glucosidase Transglucosylation Cellulase Nigerose Kojibiose イソマルトース

项目摘要

1. For the application of the transglucosylation to enzymatic syntheses of oligosaccharides, various types of <alpha> -glucosidases were purified as crystalline or homogeneous protein from brewer's yeast, Asp. niger, Asp, oryzae honey bee and buckwheat.2. New trisaccharide, <3^G> - <alpha> -glucosyl-sucrose was synthesized by the transglucosylation of immobilized buckwheat <alpha> -glucosidase.3. Di- and trisaccharides such as isomaltose and panose were prepared and isolated by crystalline Asp. niger <alpha> -glucosidase.4. Buckwheat <alpha> -glucosidase are capable of specifically producing kojibiose and nigerose. However, there has been no report on the method for simultaneous determination of kojibiose, nigerose, maltose and isomaltose in the reaction mixture. The amounts of disaccharides produced by buckwheat <alpha> -glucosidase were quantitatively determined for the first time by combination of a gas-liquid and a paper chromatographic methods. A large amount of kojibiose or nigerose was enzymatically synthesized by the technique estabished in this reaearch.5. It is usually difficult to separate nigerose from maltose. A technique by which pure nigerose can be prepared was established by use of honeybee <alpha> -glucosidase having no hydrolytic activity to nigerose. 6. A cellulase was purified from Asp. niger for the examination of the preparation method of cello-oligosaccharides.7. An isomalto-dextranase was homogeneously purified from A. globiformis for the preparation of isomaltose.

1.为了将转葡萄糖基化应用于寡糖的酶促合成，将各种类型的α-葡萄糖苷酶从啤酒酵母Asp中纯化为结晶或均质蛋白质。黑日尔、天冬氨酸、米蜜蜂和荞麦。2．通过固定化荞麦<α>-葡萄糖苷酶的转糖基作用合成了新的三糖<3^G>-<α>-葡萄糖基蔗糖。 3．通过结晶天冬氨酸制备和分离二糖和三糖，例如异麦芽糖和潘糖。尼日尔<α>-葡萄糖苷酶.4。荞麦α-葡萄糖苷酶能够特异性地产生曲二糖和黑曲二糖。但同时测定反应混合物中曲二糖、黑曲霉糖、麦芽糖和异麦芽糖的方法尚未见报道。首次采用气液和纸色谱相结合的方法定量测定了荞麦α-葡萄糖苷酶产生的二糖量。采用本研究建立的技术，酶法合成了大量的曲二糖或黑曲糖。 5．通常很难将黑糖与麦芽糖分离。通过使用对黑曲霉糖不具有水解活性的蜜蜂α-葡萄糖苷酶，建立了可制备纯黑曲霉糖的技术。 6.从Asp中纯化纤维素酶。纤维寡糖制备方法的检验。 7．从球状曲霉中均匀纯化异麦芽糖葡聚糖酶，用于制备异麦芽糖。