Small non-coding RNAs in Streptomyces coelicolor

天蓝色链霉菌中的小非编码 RNA

• 批准号: 40095809
• 负责人: Professorin Dr. Beatrix Süß
• 金额: --
• 依托单位: Fachgebiet Molecular Genetics
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/40095809?language=en
• 关键词: Small; non; coding; RNAs; Streptomyces

Streptomyces coelicolor is a Gram-positive, GC-rich soil bacterium that serves as a model organism for the investigation of secondary metabolite production (antibiotics) and cell differentiation. Our research topic is the analysis of small regulatory RNAs (sRNAs) in this organism. During the last funding period we characterized the sRNA sc1. It controls the expression of the agarase DagA by a direct interaction with the ribosomal binding site of the target mRNA. Moreover, sc1 is involved in nitrogen metabolism and influences the production of antibiotics. We aim to characterize further targets of sc1 and unravel its role in the control of secondary metabolism. We have identified further ~ 800 potential sRNAs using a deep sequencing approach and selected a set of eight sRNAs for further analysis. They are differentially expressed in minimal media and show nitrogen or temperature dependence, respectively. We will perform a detailed structural and functional characterization of these candidates. Further on, we attempt to identify proteins involved in the sRNA-mRNA interactions since S. coelicolor lacks the major chaperon Hfq which facilitates this interaction in other organisms.

天蓝色链霉菌是一种革兰氏阳性、富含 GC 的土壤细菌，可作为研究次级代谢产物（抗生素）和细胞分化的模型生物。我们的研究主题是分析该生物体中的小调节 RNA (sRNA)。在上一次资助期间，我们对 sRNA sc1 进行了表征。它通过与靶 mRNA 的核糖体结合位点直接相互作用来控制琼脂酶 DagA 的表达。此外，sc1参与氮代谢并影响抗生素的产生。我们的目标是进一步表征 sc1 的靶点并揭示其在控制次生代谢中的作用。我们使用深度测序方法进一步鉴定了约 800 个潜在 sRNA，并选择了一组 8 个 sRNA 进行进一步分析。它们在基本培养基中差异表达，并分别表现出氮或温度依赖性。我们将对这些候选物进行详细的结构和功能表征。此外，我们尝试鉴定参与 sRNA-mRNA 相互作用的蛋白质，因为天蓝色链球菌缺乏促进其他生物体中这种相互作用的主要伴侣 Hfq。