Domoic acid-binding substance found in bacteria isolated from causative diatom of domoic acid

从软骨藻酸致病硅藻中分离出的细菌中发现软骨藻酸结合物质

• 批准号: 23658175
• 负责人: KODAMA Masaaki
• 金额: $ 2.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658175/
• 关键词: ドウモイ酸; 海洋細菌; 抗ドウモイ酸抗体; 記憶喪失性貝毒; アフィニティカラム; 細菌; ベトナム

研究成果の概要（英文）：Domoic acid (DA) is a compound with anthelmintic activity which was identified for the first time in a red algae Chondria armata. On the other hand, DA was found to be the causative toxin for Amnesic Shellfish Poisoning (ASP) that was first recognized in Canada in 1978. Accumulation of DA in shellfish was observed during the bloom of Pseudo-nitzschia multiseries, Thus this species was considered to be the causative species. Soon it was confirmed by culture experiment. Now several species of Pseudo-nitzschia are considered to be the causative species. The species identification of Pseudo-nitzschia spp. is usually performed by electron microscope. Thus it is impossible to know relation between DA accumulation and bloom of toxic Pseudo-nitzschia species. We developed an ELSA for DA and tried to survey the relation between the DA level in plankton samples and that accumulated in the shellfish. Interestingly, the DA level of shellfish did not increase during when significant level of DA was observed in plankton samples. In contrast, DA of shellfish increased much around 1 week after DA in plankton samples disappeared. These facts strongly suggest the occurrence of DA-binding substance in plankton cells. -DA may be released when the DA-binding substance was digested by the shellfish. In the present study, we could show the possible occurrence of the DA-binding substance in bivalve that accumulates high level of DA.

研究结果摘要（英文）： 软骨藻酸 (DA) 是一种具有驱虫活性的化合物，首次在红藻 Chondria armata 中发现。另一方面，DA 被发现是遗忘性贝类的致病毒素。中毒 (ASP) 于 1978 年在加拿大首次被认识到。在拟菱形藻盛开期间观察到贝类中 DA 的积累，因此，很快通过培养实验证实了拟菱形藻的种类，因此通常通过电子显微镜进行鉴定。不可能知道 DA 积累与有毒拟菱形藻水华之间的关系。我们开发了 DA 的 ELSA，并尝试调查浮游生物样本中 DA 水平与水华之间的关系。与贝类中积累的情况不同，当浮游生物样品中观察到显着水平的 DA 时，贝类中的 DA 水平并没有强烈增加，相反，在浮游生物样品中的 DA 消失后 1 周左右，贝类中的 DA 增加了很多。 -当DA结合物质被贝类消化时，DA可能被释放。在本研究中，我们可以证明DA结合物质在双壳类动物中可能存在。积累高水平的DA。