Critical analyses of "Sex" in homothallic Closterium peracerosum-strigosum-littorale complex

同宗新月藻-条纹藻-滨海复合体中“性”的批判性分析

• 批准号: 23657161
• 负责人: SEKIMOTO Hiroyuki
• 金额: $ 2.5万
• 依托单位: Japan Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657161/
• 关键词: ミカヅキモ; 有性生殖; ホモタリズム; ヘテロタリズム; 性分化; 性決定; ホモタリック

In Closterium peracerosum-strigosum-littorale complex, two types of conjugation produce zygotes; one is a conjugation between two complementary mating-type cells (mt+ and mt-) and the other is a conjugation between clonal cells. The former is referred to as heterothallism and the latter as homothallism.Heterothallic mating group II-B and homothallic strains (naga37) are phylogenetically closely related. The formation of hybrid zygotes between the homothallic cells and either heterothallic cells was confirmed. These results suggest that at least some of the homothallic gametangial cells possess the same characteristics as heterothallic mt- and/ or mt+ cells. On the other hand, progenies derived from the hybrid zygotes could not be obtained.Total RNA was collected from cells in several asexual and sexual reproductive stages and large scale RNAseq data was obtained by using new generation sequencer Hiseq2000. Finally, 132329 transcripts including many isoforms were established.

在闭叶毛 - 甲状腺膜层中，两种类型的共轭产生Zygotes。一种是两个互补交配型细胞（MT+和MT-）之间的共轭，另一个是克隆细胞之间的共轭。前者被称为异义主义，后者称为同质主义。属于II-B组和同质菌株（NAGA37）在系统发育上密切相关。确认了同质细胞和任何一个异质细胞之间的杂化合子的形成。这些结果表明，至少某些同质配子式细胞具有与异性MT-和/或MT+细胞相同的特征。另一方面，无法获得从杂种合子中得出的后代。从几个无性和性生殖阶段中收集了细胞的全部RNA，并使用新一代Suepencer Sequencer Hiseq2000获得了大规模RNASEQ数据。最后，建立了包括许多同工型在内的132329个成绩单。

项目成果

植物細胞リン酸輸送機構とその進化について

植物细胞磷酸盐转运机制及其演化

• 发表时间: 2015
• 作者: 藤原ひとみ;大西美輪;坂山英俊;石崎公庸;豊倉浩一;関本弘之;西山智明;七條千津子;小菅桂子;深城英弘;三村徹郎
• 通讯作者: 三村徹郎

生殖隔離に関わる性フェロモンのゲノムレベルでの特徴

生殖隔离涉及的性信息素的基因组特征

• 发表时间: 2013
• 作者: Masuzaki;H.;Furuki;T.;Dalton;P. J.;Tsubota;H.;Seppelt;R. D. & Deguchi;H.;高橋一男;井上侑哉・坪田博美;土金勇樹;Kazuo H. Takahashi;土金勇樹・西山智明・関本 弘之
• 通讯作者: 土金勇樹・西山智明・関本 弘之

単細胞シャジクモ藻類ミカヅキモにおける生殖隔離、種分化機構の研究

单细胞蜘蛛藻Mikazukimo的生殖隔离和物种形成机制研究

• 发表时间: 2012
• 期刊: IFO Research communications
• 作者: 浜地心;楳原琢哉;田村浩二;Michihiko Ito;関本弘之
• 通讯作者: 関本弘之

Characterization of a conjugation processes in homothallic Closterium peracerosum-strigosum-littorale complex

同宗新月藻-strigosum-littorale 复合体中缀合过程的表征

• 发表时间: 2013
• 作者: Tsuchikane;Y.;Sekimoto;H.
• 通讯作者: H.

Hybrid zygospore formation between homothallic and heterothallic strains of Closterium peracerosum-strigosum-littorale complex

新月藻-条纹藻-滨海复合体的同宗和异宗菌株之间杂种接合孢子的形成

• 作者: Tsuchikane;Y.;Tsuchiya;M.;Baba;M.;Nozaki;H.;Sekimoto;H.
• 通讯作者: H.