Structural and biochemical characterisation of plant EDS1 disease resistance regulatory complexes

植物 EDS1 抗病调节复合物的结构和生化特征

• 批准号: 39953024
• 负责人: Professor Dr. Karsten Niefind
• 金额: --
• 依托单位: Institut für Biochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/39953024?language=en
• 关键词: Structural; biochemical; characterisation; plant; EDS1; Structural; biochemical; characterisation; plant; EDS1

In plants, the nucleo-cytoplasmic protein EDS1 and its interacting partners PAD4 and SAG101 control the induction of defences and programmed cell death against invasive pathogens. Here, we aim in a collaborative project to elucidate the biochemical and molecular mechanisms by which EDS1 complexes transduce signals inside the cell. Although EDS1 and its partners have sequence similarities to lipases, our genetical and biochemical data reveal that they are not acyl hydrolase enzymes. Instead, we have evidence of a lipid reducing activity by EDS1 and have identified sequence motifs that could mediate lipid binding. We have developed protocols to purify large quantitities of soluble Arabidopsis EDS1 alone or complexed with PAD4 or SAG101 from E.coli. We will use the pure proteins to determine the physico-chemical and kinetic properties of EDS1 complexes, intensify crystallisation experiments and further characterize EDS1 enzymatic and lipid binding capabilities. Data produced should provide a new dimension and direction to understanding intrinsic protein functions in the plant defence signalling network.

在植物中，核总质蛋白EDS1及其相互作用的伴侣PAD4和SAG101控制防御诱导和针对侵入性病原体的程序性细胞死亡。在这里，我们的目标是一个协作项目，旨在阐明EDS1复合物在细胞内部传统信号的生化和分子机制。尽管EDS1及其伴侣与脂肪酶具有序列相似性，但我们的遗传和生化数据表明它们不是酰基水解酶。取而代之的是，我们有EDS1脂质还原活性的证据，并确定了可以介导脂质结合的序列基序。我们已经开发了单独纯化大量可溶性拟南芥EDS1或与E.Coli的PAD4或SAG101复合的方案。我们将使用纯蛋白来确定EDS1复合物的物理化学和动力学特性，增强结晶实验，并进一步表征EDS1酶促和脂质结合能力。产生的数据应提供一个新的维度和方向，以理解植物防御信号网络中的内在蛋白质功能。