Small regulatory RNAs from the halophilic archaeon Haloferax volcanii

来自嗜盐古菌 Haloferax volcanii 的小调控 RNA

• 批准号: 39788743
• 负责人: Professorin Dr. Anita Marchfelder
• 金额: --
• 依托单位: Institut für Molekularbiologie und Biotechnologie der Prokaryoten
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/39788743?language=en
• 关键词: Small; regulatory; RNAs; halophilic; archaeon

While the function and mechanism of small regulatory RNAs in Bacteria and Eukarya have been analysed in detail, not much is known about the occurence and function of regulatory RNAs in the third domain of life, the Archaea. In the last funding period, we identified the small RNA (sRNA) population of the halophilic archaeon Haloferax volcanii with three different experimental and two bioinformatic methods. Taken together more than 200 potential regulatory RNAs were identified. In addition, we could show that deletion of sRNA genes is feasible and in most cases not lethal. In the next funding period, our main goal is to identify targets of the identified potential regulatory sRNAs. This will allow the characterisation of the sRNA-target interaction in detail and to identify the mechanism of archaeal sRNA regulation.

虽然细菌和真核生物中小调节RNA的功能和机制已经被详细分析，但对于生命第三个领域——古细菌中调节RNA的出现和功能知之甚少。在上一个资助期间，我们通过三种不同的实验和两种生物信息学方法鉴定了嗜盐古菌 Haloferax volcanii 的小 RNA (sRNA) 种群。总共鉴定出了 200 多种潜在的调控 RNA。此外，我们可以证明删除 sRNA 基因是可行的，并且在大多数情况下不是致命的。在下一个资助期内，我们的主要目标是确定已确定的潜在监管 sRNA 的靶标。这将能够详细描述 sRNA-靶标相互作用的特征，并确定古菌 sRNA 调节的机制。