Localization and regulation in reactive sites of DAMPs/PAMPs. Role of ATP from the activated plateles

DAMPs/PAMPs 反应位点的定位和调节。

• 批准号: 25670764
• 负责人: MARUYAMA Ikuro
• 金额: $ 2.41万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25670764/
• 关键词: ATP; DAMP; PAMP; platelet; inflammation; PAMPｓ; DAMPｓ; ＡＴＰ; Ａｄｅｎｏｓｉｎｅ; Inflammasome; IL-1β; IL-18; ATP; DAMP; PAMP; platelet; inflammation; PAMPｓ; DAMPｓ; ＡＴＰ; Ａｄｅｎｏｓｉｎｅ; Inflammasome; IL-1β; IL-18

Dual signalings from PAMPs/DAMPs play crucial role in inflammation, immunity and hemostasis. It has been listed more than 1000 of molecules as PAMPs, and DAMPs each other.In this study, we took LPS as PAMPs,and HMGB-1 and ATP as DAMPs, and stimulated macrophages or dendritic cells. We showed that dual signaling from LPS and HMGB1 or ATP stimulated the release of IL-1b(beta) and IL-18.Thus dual signaling plays crucial role in inflammation, immunity and hemostasis. It has been described that platelet contains inflammasome, however the role of platelet-inflammasome has been not known. We examined the role of platelet inflammasome. An inflammasome inhibitor,1,5-anhydrofructose inhibited platelets aggregation with decreased release of IL-1b(beta). Thus ATP from activated platelets might play important role for the hemostasis/inflammation.

小型/潮湿的双重信号在炎症，免疫和止血中起着至关重要的作用。它已被列为1000多个分子作为大小型的分子，并互相潮湿。在这项研究中，我们将LPS作为PAMPs，HMGB-1和ATP作为潮湿，并刺激了巨噬细胞或树突状细胞。我们表明，来自LPS和HMGB1或ATP的双重信号刺激了IL-1B（beta）和IL-18的释放。已经描述了血小板含有炎症体，但是血小板 - 炎症体的作用尚不清楚。我们检查了血小板炎性体的作用。炎性体抑制剂，1,5-芳基果糖抑制了血小板聚集，而IL-1B释放降低（beta）。因此，活化血小板的ATP可能对止血/炎症起重要作用。