Establishment of salivary gland regeneration method using salivary gland tissue-originated spheroid (STOS)

利用唾液腺组织源性球体（STOS）建立唾液腺再生方法

• 批准号: 24659899
• 负责人: SUGIURA Tsuyoshi
• 金额: $ 2.41万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659899/
• 关键词: 幹細胞; 唾液腺再生; Brachyury; 唾液腺; 再生医療

We demonstrated the regenerative capability of dissociated E13.5 embryonic submandibular gland (SMG) cells in vitro in collagen type I gels. The resulting structures were similar to native salivary glands. Large cyst-like structures, probably due to swollen ducts, began to appear on day 7, and they enlarged and increased in number by day 14. Through the addition of fibronectin and an anti- integrin antibody into the regenerate salivary glands, cyst formation and enlargement were clearly inhibited, and the expression and localization of AQP5 was concentrated on the lumen side with cell polarity similar to native salivary glands. In contrast, anti- integrin antibodies induced cyst formation and enlargement. Together, these data indicate that dissociated SMGs can regenerate salivary gland structure, and specific interactions between integrins and extracellular matrices are essential for regenerate salivary gland differentiation and development.

我们在体外 I 型胶原凝胶中证明了分离的 E13.5 胚胎颌下腺 (SMG) 细胞的再生能力。由此产生的结构与天然唾液腺相似。 可能是由于导管肿胀而导致的大囊肿样结构在第 7 天开始出现，并在第 14 天增大且数量增加。通过在再生唾液腺中添加纤连蛋白和抗整合素抗体，囊肿形成和数量增加。增大明显受到抑制，AQP5的表达和定位集中在管腔侧，细胞极性与天然唾液腺相似。 相反，抗整联蛋白抗体诱导囊肿形成和扩大。 总之，这些数据表明解离的 SMG 可以再生唾液腺结构，整合素和细胞外基质之间的特定相互作用对于再生唾液腺分化和发育至关重要。