Utility value of blastocyst biopsy for preimplantation genetic diagnosis

囊胚活检在植入前遗传学诊断中的实用价值

• 批准号: 24659739
• 负责人: TOMIYAMA Ryoko
• 金额: $ 2.41万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659739/
• 关键词: 胚盤胞生検; 分裂期; 胚盤胞; 均衡型転座; 習慣流産; 着床前診断

Embryo biopsy intended for preimplantation genetic diagnosis (PGD) is generally performed on cleavage stage, but it tend to be harmful for embryo viability. Trophectoderm biopsy from blastocyst is seem to be less invasive, since inner cell mass is remain intact after biopsy. In this study it could be easily biopsied several number of trophectoderm cells and all of blastocysts expanded again after biopsy. Some blastocyst cells treated by Colcemid were significantly interrupted mitosis, which drug efficacy was confirmed by Hoechst and Propidium iodide staining. On the other hand, fluorescent signals of whole chromosome painting probes were strongly detectable as for lymphocyte FISH. Trophectoderm biopsiy followed by examination using mitotic stage cells was likely to useful for PGD.

用于植入前遗传学诊断（PGD）的胚胎活检通常在卵裂阶段进行，但往往对胚胎活力有害。来自囊胚的滋养外胚层活检似乎侵入性较小，因为活检后内细胞团保持完整。在这项研究中，可以很容易地对多个滋养外胚层细胞进行活检，并且所有囊胚在活检后再次扩张。 Colcemid处理的一些囊胚细胞有丝分裂明显中断，其药效通过Hoechst和碘化丙啶染色得到证实。另一方面，与淋巴细胞 FISH 一样，可以强烈检测到全染色体涂色探针的荧光信号。 滋养外胚层活检，然后使用有丝分裂期细胞进行检查，可能对 PGD 有用。

项目成果

Gonadotropin surge stimulate ovarian osteopontin expression via epidermal growth factor receptor signaling to enhance corpus luteum function

促性腺激素激增通过表皮生长因子受体信号传导刺激卵巢骨桥蛋白表达，增强黄体功能

• 发表时间: 2013
• 作者: Yoshimitsu Kuwabara;Akira Katayama;Ryoko Tomiyama;Syuichi Ono;Katsuya Mine;Toshiyuki Takeshita
• 通讯作者: Toshiyuki Takeshita