Induction of keratinocyte stem cell based on epidermal transcription factors

基于表皮转录因子的角质形成细胞干细胞诱导

• 批准号: 24659519
• 负责人: ISHII Yoshiyuki
• 金额: $ 2.41万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659519/
• 关键词: 角化細胞; 転写因子; 分化; ケラチノサイト; 角化

PTTG1 is the transcription factor involved in the control of cell growth, differentiation, and apoptosis. The present study was performed to examine the role of PTTG1 in proliferation and differentiation of keratinocytes with formation of a stratified epidermoid structure in air-liquid interface 3-dimensional culture. PTTG1 was expressed specifically in undifferentiated normal keratinocytes and downregulated during differentiation. The constitutive overexpression of PTTG1 in keratinocytes during air exposure-induced differentiation resulted in an undifferentiated phenotype, i.e., thickening of the stratified layers, suppression of differentiation marker expression, and retention of proliferative activity with activation of cell cycle regulatory proteins in the S and G2/M phases. These findings suggested that PTTG1 may play an important role in maintenance of the undifferentiated phenotype of keratinocytes.

PTTG1是控制细胞生长，分化和凋亡的转录因子。进行了本研究以检查PTTG1在角质形成细胞增殖和分化中的作用，并在空气界面3维培养中形成了表皮样结构。 PTTG1在未分化的正常角质形成细胞中特别表达，并在分化过程中下调。在空气暴露诱导的分化过程中，PTTG1在角质形成细胞中的组成型过表达导致未分化的表型，即分层层的增厚，抑制分化标记物表达以及通过SS和G2/M M层中细胞周期调节蛋白激活的激活来抑制分化标记的表达。这些发现表明，PTTG1可能在维持角质形成细胞的未分化表型中起重要作用。

项目成果

Linear lupus erythematosus profundus involving the muscle with an intractable deep ulcer, successfully treated with surgical excision

累及肌肉的线状深部红斑狼疮，伴有顽固性深部溃疡，通过手术切除成功治愈

• DOI: 10.1684/ejd.2013.2068
• 发表时间: 2013
• 期刊: Eur J Dermatol
• 影响因子: 2.5
• 作者: Nakamura Y;Kawachi Y;Nakamura Y;Iino Y;Fujisawa Y;Otsuka F.
• 通讯作者: Otsuka F.

Docetaxel was effective in the management of metastatic angiosarcoma associated with disseminated intravascular coagulation syndrome

多西紫杉醇可有效治疗与弥散性血管内凝血综合征相关的转移性血管肉瘤

• DOI: 10.1111/j.1365-4632.2012.05671.x
• 发表时间: 2014
• 期刊: Int J Dermatol
• 影响因子: 3.6
• 作者: Fujisawa Y;Nakamura Y;Kawachi Y;Otsuka F.
• 通讯作者: Otsuka F.

Incidental gastric signet-ring cell carcinoma metastasis to the skin in basal cell carcinoma.

基底细胞癌中胃印戒细胞癌偶然转移至皮肤。

• DOI: 10.2340/00015555-1295
• 发表时间: 2012
• 期刊: Acta Derm Venereol
• 作者: Kawachi Y;Fujisawa Y;Furuta J;Nakamura Y;Ishii Y;Otsuka F.;Nakamura Y et al
• 通讯作者: Nakamura Y et al

Indocyanine green fluorescence-navigated sentinel node biopsy showed higher sensitivity than the radioisotope or blue dye method, which may help to reduce false-negative cases in skin cancer

• DOI: 10.1002/jso.23045
• 发表时间: 2012-07-01
• 期刊: JOURNAL OF SURGICAL ONCOLOGY
• 影响因子: 2.5
• 作者: Fujisawa, Yasuhiro;Nakamura, Yasuhiro;Otsuka, Fujio
• 通讯作者: Otsuka, Fujio

Malignant blue nevus arising in a giant congenital cellular blue nevus in an infant.

婴儿巨大先天性细胞蓝痣中出现的恶性蓝痣。

• DOI: 10.1111/j.1525-1470.2011.01662.x
• 发表时间: 2012
• 期刊: Pediatr Dermatol
• 影响因子: 1.5
• 作者: Kawachi Y;Fujisawa Y;Furuta J;Nakamura Y;Ishii Y;Otsuka F.;Nakamura Y et al;Nakamura Y et al
• 通讯作者: Nakamura Y et al