Influence of mechanical stress applied to ES/iPS cells on organelle control and cell metabolism/differentiation

ES/iPS 细胞机械应力对细胞器控制和细胞代谢/分化的影响

• 批准号: 24659454
• 负责人: ITOH Hiroshi
• 金额: $ 2.41万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659454/
• 关键词: ES細胞; 細胞分化; 腎臓細胞; メカニカルストレス

Our objective was to establish a method for inducing the differentiation of renal tubular cells from human ES cells, and to clarify the significance of mechanical stress in the process of differentiation. KSP was used as a marker for renal tubular cell differentiation. Differentiation to an intermediate mesoderm was promoted using GSK3β inhibitor. Epithelization was then promoted using a reduced serum medium containing EGF, which successfully yielded approximately 5% KSP-positive cells. We then investigated the influence of mechanical stress on cell differentiation using HKC-8, a human proximal tubular cell, under different conditions. However, it was not possible to apply sufficient mechanical stress. We are therefore planning to conduct further investigations in the future.

我们的目的是建立一种诱导人ES细胞分化为肾小管细胞的方法，并阐明机械应力在分化过程中作为肾小管细胞分化为中间体的标志物的意义。使用 GSK3β 抑制剂促进上皮化，然后使用含有 EGF 的减少血清培养基促进上皮化，成功地产生了大约 5% 的 KSP 阳性细胞，然后我们使用 HKC-8（一种）研究了机械应力对细胞分化的影响。然而，我们计划在未来进行进一步的研究。

项目成果

ヒトES細胞を用いた尿細管上皮細胞への分化誘導の試み

尝试使用人ES细胞诱导分化为肾小管上皮细胞

• 发表时间: 2013
• 作者: 中津祐介;岩下未咲;松永泰花;福嶋俊明;迫田秀之;櫛山暁史;鎌田英明;内田隆史;浅野知一郎;Suganami T;山口慎太郎
• 通讯作者: 山口慎太郎

ヒトES細胞を用いた尿細管上皮細胞の分化誘導の試み

尝试使用人ES细胞诱导肾小管上皮细胞分化

• 发表时间: 2013
• 作者: 山口慎太郎;本間康一郎;門川俊明;森実隆司;鈴木さゆり;藤井静花;脇野修;林晃一;伊藤裕
• 通讯作者: 伊藤裕