Engineering of a hydrocarbon producing yeast for biofuel production

用于生物燃料生产的碳氢化合物生产酵母的工程

• 批准号: 24658289
• 负责人: KAGIWADA Satoshi
• 金额: $ 2.16万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658289/
• 关键词: バイオ燃料; 緑藻; 酵母; 炭化水素; バイオテクノロジー; 再生可能エネルギー; 微生物

A unicellular green algae, Botryococcus braunii, secretes a hydrocarbon called botryococcene, which is very suitable for biofuel production, into its environment. However, Botryococcus do not grow fast and is not subjected to biotechnological engineering, making it difficult to improve this organism for more efficient biofuel production.In this study, genes required for botryococcene synthesis are incorporated into the budding yeast Saccharomyces cerevisiae, which is very suitable for biotechnological engineering, to make a yeast strain which can prouduce large amounts of botryococcene and secrete them.

单细胞绿藻（Botryocococcus braunii）分泌一种称为氢氯环苯烯的碳氢化合物，非常适合生产生物燃料的生产。 However, Botryococcus do not grow fast and is not subjected to biotechnological engineering, making it difficult to improve this organism for more efficient biofuel production.In this study, genes required for botryococcene synthesis are incorporated into the budding yeast Saccharomyces cerevisiae, which is very suitable for biotechnological engineering, to make a yeast strain which can prouduce large amounts of botryococcene and分泌他们。

项目成果

Biosynthesis and accumulation of lipids and polysaccharides in a colonial green alga, Botryococcus braunii race B

群体绿藻布氏葡萄球菌 B 种脂质和多糖的生物合成和积累

• 发表时间: 2013
• 作者: Uno;Y.;Suzuki;R.;Ito;N.;Kagiwada;S.;Nishii;I. and Noguchi;T.
• 通讯作者: T.

緑藻Botryococcus braunii Ｂ品種における脂質の分泌に伴うオイルボディの動態

绿藻布氏葡萄球菌 B 品种中与脂质分泌相关的油体动态

• 作者: Tadashi Nakai;Takafumi Deguchi;Ivo Frebort;Katsuyuki Tanizawa;Toshihide Okajima.;鈴木玲子,宇野由紀,鍵和田聡,西井一郎,野口哲子
• 通讯作者: 鈴木玲子,宇野由紀,鍵和田聡,西井一郎,野口哲子

緑藻Botryococcus brauniiのトリテルペン合成酵素群の出芽酵母での発現と細胞内局在

绿藻布氏葡萄球菌三萜合酶组在酿酒酵母中的表达和细胞内定位

• 发表时间: 2013
• 作者: 三井菜々香;野口哲子;鍵和田聡
• 通讯作者: 鍵和田聡

Biosynthesis and accumulation of hydrocarbons and polysaccharides in a colonial green alga, Botryococcus braunii race B

殖民地绿藻布氏葡萄球菌 B 种碳氢化合物和多糖的生物合成和积累

• 发表时间: 2013
• 作者: Suzuki;R.;Uno;Y.;Nishii;I.;Kagiwada;S. and Noguchi;T.
• 通讯作者: T.

Transformation of lipid bodies related to hydrocarbon accumulation in a green alga, Botryococcus braunii (Race B).

• DOI: 10.1371/journal.pone.0081626
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Suzuki R;Ito N;Uno Y;Nishii I;Kagiwada S;Okada S;Noguchi T
• 通讯作者: Noguchi T