Involvement of maternal mRNA in the regulation of differentiation during preimplantation development.

母体 mRNA 参与植入前发育过程中分化的调节。

• 批准号: 24658244
• 负责人: AOKI Fugaku
• 金额: $ 2.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658244/
• 关键词: 初期発生; 細胞分化; 母性mRNA; 2細胞期胚; 4細胞期胚; 初期胚; 発生; 分化; 卵母細胞; 卵

To identify the maternal mRNA that are involved in the differentiation during preimplantation development, I conducted RNA sequencing analysis using the oocytes and embryos. The results showed that although 70% of maternal mRNAs were degraded soon after fertilization, the rest of them survived this wave of a rapid degradation. Especially, those from 691 genes were hardly degraded during the 1-cell stage. Gene Ontology (GO) enrichment analysis revealed that these genes were enriched in the GO terms categorized into transcription.

为了鉴定参与植入前发育过程分化的母体 mRNA，我使用卵母细胞和胚胎进行了 RNA 测序分析。结果显示，虽然70%的母体mRNA在受精后很快就被降解，但其余的mRNA在这波快速降解中幸存下来。尤其是来自691个基因的基因在1细胞阶段几乎没有被降解。基因本体 (GO) 富集分析显示，这些基因在分类为转录的 GO 术语中富集。

项目成果

マウス受精前後における母性mRNA分解に関する網羅的な動態解析

小鼠受精前后母体mRNA降解的综合动力学分析

• 发表时间: 2013
• 作者: 金綾奈;鈴木元;阿部健一郎;青木不学
• 通讯作者: 青木不学

RNAシーケンスによるマウス一細胞期胚の遺伝子発現解析

通过 RNA 测序对小鼠单细胞阶段胚胎进行基因表达分析

• 发表时间: 2013
• 作者: 阿部健一郎;山本龍馬;曹旻君;鈴木穣;青木不学
• 通讯作者: 青木不学