A metabolic futile cycle is operating in gas gland cells of the swimbladder.

鳔的气腺细胞中正在进行代谢无效循环。

• 批准号: 24657085
• 负责人: HIROSE Shigehisa
• 金额: $ 2.5万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657085/
• 关键词: 酵素の調節; 代謝調節; ルート効果ヘモグロビン; 解糖系; 乳酸輸送体; Fructose bisphosphatase; 空転サイクル; 熱発生; 対向流血管系; ガス腺

H+/monocarboxylate transporters MCT1b and MCT4b were found to be highly expressed in the fugu swimbladder. Immunohistochemical analyses of metabolic enzymes demonstrated that a gluconeogenesis enzyme fructose-1,6-bisphosphatase (Fbp1) and a glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (Gapdh) are highly expressed in gas gland cells. The simultaneous catalyses of glycolysis and gluconeogenesis reactions suggest the presence of a futile cycle in gas gland cells to maintain the levels of ATP low and to generate heat that helps reduce the solubility of O2

发现 H+/单羧酸转运蛋白 MCT1b 和 MCT4b 在河豚鳔中高度表达。代谢酶的免疫组织化学分析表明，糖异生酶果糖-1,6-二磷酸酶（Fbp1）和糖酵解酶甘油醛-3-磷酸脱氢酶（Gapdh）在气腺细胞中高度表达。糖酵解和糖异生反应的同时催化表明气腺细胞中存在无效循环，以维持较低的 ATP 水平并产生有助于降低 O2 溶解度的热量

项目成果

Histological demonstration of glucose transporters, fructose-1, 6-bisphosphatase, and glycogen in gas gland cells of the swimbladder : is a metabolic futile cycle operating

鱼鳔气腺细胞中葡萄糖转运蛋白、果糖-1、6-双磷酸酶和糖原的组织学证明：是一个代谢无效循环运行

• DOI: 10.1016/j.bbrc.2011.12.006
• 发表时间: 2012
• 期刊: Biochem Biophys Res Commun
• 作者: Munakata K;Ookata K;Doi H;Baba O;Terashima T;Hirose S;Kato A.
• 通讯作者: Kato A.