Study on the mechanism of tissue disruption induced by decreased expression of cell-cell adhesion proteins

细胞-细胞粘附蛋白表达减少引起组织破坏机制的研究

• 批准号: 24656175
• 负责人: AOMURA Shigeru
• 金额: $ 2.58万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24656175/
• 关键词: 毛細血管; 細胞間接着タンパク質; 衝撃圧力; 組織崩壊; 脳内点状出血; 頭部外傷; 脳挫傷; 血管内皮細胞; 経内皮電気抵抗値; 血管透過性; 密着結合

Intracranial pressure changes during head impact cause brain injuries such as vasogenic edema and cerebral contusion.Human umbilical vein endothelial cells were subjected to positive and negative pressure, and the transendothelial electrical resistance (TEER) was measured between 15 min and 24 h after pressure loading for quantifying the formation of an integral monolayer of endothelial cells.TEER decreased considerably at 15 min and 6 h post-loading, with these changes being significant in positive pressure with larger amplitude and shorter duration and in all types of negative pressures compared to pre-loading.The changes in intracranial pressure during head impact impair endothelial barrier function by the disruption of the integrity of endothelial cell-cell junctions, and the degree of increase in endothelial permeability depends on the amplitude, duration, and direction (compressive and tensile) of the impulsive pressure.

Intracranial pressure changes during head impact cause brain injuries such as vasogenic edema and cerebral contusion.Human umbilical vein endothelial cells were subjected to positive and negative pressure, and the transendothelial electrical resistance (TEER) was measured between 15 min and 24 h after pressure loading for quantifying the formation of an integral monolayer of endothelial cells.TEER decreased considerably at 15 min and 6 h post-loading, with these changes being significant in positive pressure with larger amplitude and shorter duration and in all types of negative pressures compared to pre-loading.The changes in intracranial pressure during head impact impair endothelial barrier function by the disruption of the integrity of endothelial cell-cell junctions, and the degree of increase in endothelial permeability depends on the amplitude, duration, and direction (compressive and tensile) of the冲动压力。

项目成果

衝撃圧力が管腔形成された血管内皮細胞に与える影響

冲击压力对管腔形成血管内皮细胞的影响

• 发表时间: 2014
• 作者: Nakadate;H.;Inuzuka;K.;Akanuma;S.;Kakuta;A. and Aomura;S;青村 茂;Hiromichi NAKADATE,Hiroto UMAHASHI,Akira KAKUTA and Shigeru AOMURA;大津英理子,青村茂,中楯浩康,角田陽
• 通讯作者: 大津英理子,青村茂,中楯浩康,角田陽

神経突起の伸長方向に対する衝撃ひずみ損傷評価

神经突延伸方向的冲击应变损伤评估

• 发表时间: 2012
• 作者: Nakadate;H;Akanuma;S;Aomura;S;and Kakuta A;金子由磨
• 通讯作者: 金子由磨

in vitro BBBモデルにおける衝撃圧力負荷後の経内皮電気抵抗値測定

体外 BBB 模型中冲击压力加载后的跨内皮电阻测量

• 发表时间: 2013
• 作者: Nakadate;H.;Inuzuka;K.;Akanuma;S.;Kakuta;A. and Aomura;S;青村 茂;Hiromichi NAKADATE,Hiroto UMAHASHI,Akira KAKUTA and Shigeru AOMURA;大津英理子,青村茂,中楯浩康,角田陽;川股理紗,青村茂,中楯浩康
• 通讯作者: 川股理紗,青村茂,中楯浩康

Impact pressure increases permeability of cultured human endothelial cells

冲击压力增加培养的人内皮细胞的渗透性

• 发表时间: 2012
• 作者: Nakadate;H;Akanuma;S;Aomura;S;and Kakuta A
• 通讯作者: and Kakuta A

頭部損傷メカニズムの解明ー頭を強打すると何が起きるのかー

阐明头部受伤的机制——头部受到撞击时会发生什么

• 发表时间: 2012
• 期刊: バイオメカニズム学会誌
• 作者: Nakadate;H.;Inuzuka;K.;Akanuma;S.;Kakuta;A. and Aomura;S;青村 茂
• 通讯作者: 青村 茂