Synthesis of Apatite Nanocrystals Containing Chlorophyll Molecules and the Antibody Immobilization towards Early Cancer Diagnosis

含叶绿素分子的磷灰石纳米晶的合成及抗体固定化用于早期癌症诊断

• 批准号: 24651133
• 负责人: TAGAYA MOTOHIRO
• 金额: $ 2.16万
• 依托单位: Nagaoka University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24651133/
• 关键词: 生体親和性材料; 細胞可視化技術; アパタイト; メソポーラスシリカ; クロロフィル; フルオレセイン; ナノバイオテクノロジー; 無機／有機ハイブリッド材料; 細胞可視化材料; 核形成; 希土類イオン; バイオイメージング; 診断; 発光; 希土類元素; 色素; 水酸アパタイト

The detection technique in which cancer cells at early stages can be specifically imaged by highly-biocompatible marker materials has been desired. In this study, biocompatible luminescent inorganic/organic hybrid nanoparticles were prepared in order to detect smaller tumor locations at the cellular sizes. As a result, nucleation reaction of inorganic apatite (or titania) effectively occurred on the end groups of highly-biocompatible dye molecules to successfully obtain the novel hybrid nanoparticles. The ligand molecules that can selectively react with cancer cells were chemically immobilized on the hybrid nanoparticles. The immobilized nanoparticles exhibited nontoxicity and specifically bound to cancer cells to resultantly induce intense orange (or green) luminescence. The hybrid nanoparticles that effectively work on cancer cells will provide novel concepts for non-invasive bioimaging as well as drug therapy for cancer diseases.

需要使用高度生物相产的标记材料专门成像的检测技术。在这项研究中，制备了生物相容性发光无机/有机杂交纳米颗粒，以检测细胞尺寸的较小肿瘤位置。结果，无机磷灰石（或二氧化钛）的成核反应有效地发生在高度生物相容的染料分子的末端组上，以成功地获得新型的杂化纳米颗粒。可以选择性地与癌细胞反应的配体分子化学固定在杂化纳米颗粒上。固定的纳米颗粒表现出无毒的性，并特别与癌细胞结合，从而诱导强烈的橙色（或绿色）发光。有效地在癌细胞上起作用的杂种纳米颗粒将为非侵入性生物成像以及用于癌症疾病的药物治疗提供新颖的概念。

项目成果

Control of Adhesion of Human Induced Pluripotent Stem Cells to Plasma-patterned Polydimethylsiloxane Coated with Vitronectin and gamma-globulin

人诱导多能干细胞对玻连蛋白和丙种球蛋白包被的等离子体图案化聚二甲基硅氧烷的粘附控制

• DOI: 10.1016/j.jbiosc.2014.02.009
• 发表时间: 2014
• 期刊: Journal of Bioscience and Bioengineering
• 影响因子: 2.8
• 作者: K. Shiba; M. Tagaya; N. Hanagata;Ryotaro Yamada; Koji Hattori; Saoko Tachikawa; Motohiro Tagaya (本研究代表者); Toru Sasaki; Shinji Sugiura; Toshiyuki Kanamori; Kiyoshi Ohnuma
• 通讯作者: Ryotaro Yamada; Koji Hattori; Saoko Tachikawa; Motohiro Tagaya (本研究代表者); Toru Sasaki; Shinji Sugiura; Toshiyuki Kanamori; Kiyoshi Ohnuma

Effect of Ultrasound on FT-IR Spectra of Poly (Acrylic Acid-co-N-Hydroxyethylacrylamide) in Aqueous Solution

超声波对水溶液中聚(丙烯酸-共-N-羟乙基丙烯酰胺) FT-IR 光谱的影响

• 发表时间: 2013
• 作者: S. Motozuka;M. Tagaya;T. Ikoma;T. Yoshioka;Z. Xu;J. Tanaka;Takaomi Kobayashi; Karla Lizette Tovar-Carrillo; Kazuki Nakasone; Motohiro Tagaya (本研究代表者);Ryotaro Yamada; Koji Hattori; Motohiro Tagaya (本研究代表者); Toru Sasaki; Shinji Sugiura; Toshiyuki Kanamori; Kiyoshi Ohnuma;Takaomi Kobayashi; Josue Addiel Venegas-Sanchez; Kohsuke Hirano; Motohiro Tagaya (本研究代表者);Kohtaroh Nakamoto; Ayaya Mikazuki; Masaru Ohshiro; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi;Josue Addiel Venegas-Sanchez; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi
• 通讯作者: Josue Addiel Venegas-Sanchez; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi

Preparation of Aminopropylsilane-immobilized Luminescent Hydroxyapatite Nanocrystals

氨丙基硅烷固定发光羟基磷灰石纳米晶的制备

• 发表时间: 2012
• 作者: Takeda;R.; Tagaya;M.; Ikoma;T.; Yoshioka;T.; Tanaka;J.
• 通讯作者: J.

Effect of Crosslinking Degrees on the Denaturation Temperature of Scaffold Membranes Prepared by from Fish Collagen

交联度对鱼胶原蛋白支架膜变性温度的影响

• 发表时间: 2012
• 作者: Xu;Z.; Ikoma;T.; Yoshioka;T.; Tagaya;M. (本申請者); Motozuka;S.; Uemura;T.; Tanaka;J.
• 通讯作者: J.

High Frequency Sono-Treatment on Regeneration of Agave Tequilana Waste Fibers for Heavy Metal Ion Adsorbents

高频声处理再生龙舌兰龙舌兰废纤维用于重金属离子吸附剂

• 发表时间: 2013
• 作者: S. Motozuka;M. Tagaya;T. Ikoma;T. Yoshioka;Z. Xu;J. Tanaka;Takaomi Kobayashi; Karla Lizette Tovar-Carrillo; Kazuki Nakasone; Motohiro Tagaya (本研究代表者);Ryotaro Yamada; Koji Hattori; Motohiro Tagaya (本研究代表者); Toru Sasaki; Shinji Sugiura; Toshiyuki Kanamori; Kiyoshi Ohnuma;Takaomi Kobayashi; Josue Addiel Venegas-Sanchez; Kohsuke Hirano; Motohiro Tagaya (本研究代表者);Kohtaroh Nakamoto; Ayaya Mikazuki; Masaru Ohshiro; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi;Josue Addiel Venegas-Sanchez; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi;Karla Lizette Tovar-Carrillo; Motohiro Tagaya (本研究代表者); Takaomi Kobayashi;Kohsuke Hirano; Motohiro Tagaya (本研究代表者); Satoshi Sugita Sueyoshi; Takaomi Kobayashi
• 通讯作者: Kohsuke Hirano; Motohiro Tagaya (本研究代表者); Satoshi Sugita Sueyoshi; Takaomi Kobayashi