Structure and function of hormones controlling diapause of pharate first-instar larvae in the wild silkmoth, Antheraea yamamai.

控制野生蚕蛾 Antheraea yamamai 甲虫一龄幼虫滞育的激素的结构和功能。

• 批准号: 05454063
• 负责人: SUZUKI Koichi
• 金额: $ 4.35万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454063/
• 关键词: Pharate first-instar larva; Diapause control; Repressive factor; Maturation factor; Hormone structure; Antheraea yamamai; 前幼虫態休眠; 免疫組織化学

We have examined the structure and function of two hormones controlling diapause of pharate first-instar in the wild silkmoth, Antheraea yamamai. Consequently, we obtained some significant results as follows :1.Repressive factor (RF) localized in the region of mesothorax is lost by the stimulus of chilling for long period as well as in the application of KK-42.2.Maturation factor (MF) is not identical with eclosion hormone according to the immunohistochemical analysis.3.We established a new method of bioassay for MF and purified partially this hormone.4.We found that MF activity is also present in the hemolymph of 5th instar larvae besides pre-larvae.5.We indicated that RF and MF are new peptide hormones in insect and they are not different with FXPRLamide family.

我们研究了控制野生蚕蛾 Antheraea yamamai 中丝虫一龄滞育的两种激素的结构和功能。因此，我们得到了一些有意义的结果：1.长期寒冷的刺激以及KK-42.2的应用，导致位于中胸区域的抑制因子（RF）丢失。成熟因子（MF）为免疫组织化学分析显示与羽化激素不完全相同。3.我们建立了一种新的MF生物测定方法，并部分纯化了该激素。4.我们发现MF活性也存在于羽化激素中。 5龄幼虫除前幼虫外的血淋巴。5.我们表明RF和MF是昆虫中新的肽类激素，与FXPRLamide家族没有什么不同。

项目成果

鈴木幸一: "イミダゾール系化合物による昆虫の休眠制御" 化学と生物. (発表予定)32. (1994)

Koichi Suzuki：“咪唑化合物控制昆虫滞育”，化学与生物学（演示文稿安排）32。（1994）

鈴木幸一,桑野栄一: "イミダゾール系化合物による昆虫の休眠制御" 化学と生物. 32. 221-228 (1994)

Koichi Suzuki、Eiichi Kuwano：“咪唑化合物控制昆虫滞育”，化学与生物学，32. 221-228 (1994)。

Suzuki, K., Kumagai, T., Naya, S.and Kuwano, E.: "Effect of chilling on diapause termination of pharate first-instar larvae in the wild silkmoth, Antheraea yamamai." Int.J.Wild Silkimoth & Silk. 1. 60-64 (1994)

Suzuki, K.、Kumagai, T.、Naya, S. 和 Kuwano, E.：“低温对野生蚕蛾 Antheraea yamamai 中的甲虫一龄幼虫滞育终止的影响。”

Naya,S.,Sagisaka,A.and Suzuki,K.: "Identification of a maturation factor inducing post-diapause development in pharate first-instar larvae of the wild silkmoth," Int.J.Wild Silkmoth & Silk. 1. 195-200 (1994)

Naya,S.、Sagisaka,A. 和 Suzuki,K.：“诱导野生蚕蛾的噬菌体一龄幼虫滞育后发育的成熟因子的鉴定”，Int.J.Wild Silkmoth

Naya, S., Suzuki, K., Fugo, H.and Sehnal, F.: "Eclosion hormone-like immunoreactivity in the nervous system of Bombyx mori (Lepidoptera : Bombycidae) and Antheraea yamamai (Lepidoptera : Saturniidae) before and after hatching." Eur.J.Entomol.91. 189-196 (

Naya, S.、Suzuki, K.、Fugo, H. 和 Sehnal, F.：“家蚕（鳞翅目：家蚕科）和山蚕（鳞翅目：土星科）孵化前后神经系统中的羽化激素样免疫反应性