EFECT OF COMPONENTS OF HYDROXYAPATITE ON OSTEOCONDUCTION

羟基磷灰石成分对骨传导的影响

• 批准号: 05454542
• 负责人: SHIMADA Keikichi
• 金额: $ 4.1万
• 依托单位: KOBE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454542/
• 关键词: hydroxyapatite (HAP); osteoconduction; multi-nuclear granules; open pore; culture experiment; biocompatibility; MC3T3-E1; Mg; MAP顆粒; 骨伝導能; 骨原性細胞; MC3T3-E1細胞; 培養; トリチウムチミジン; HAP顆粒; 化学組成; 家兎脛骨; 1200℃

A variety of hydroxyapaite (HAP) granules were made which have various chemical compositions, shapes and solubility behaviors, and the effects of these properties on the osteoconduction were examined. HAP powders were synthesized by precipitation method, granulated and sintered at 1200ﾟC.These results suggested that the shapes of HAP granules were closely connected with osteoconduction. Namely multi-nuclear HAP granules with continuos pores ranging from 30 mum to 200 mum in diameter and fine particle surface indicated yhe most excellent osteoconduction. This sample, also, indicated satisfactory biocompatibility to L929 culture cells. It was considered that osteoconductions of HAP granules were influenced by variation of calcium or phoshorous solubility, and then the effects of solubility behavior were examined. As the result, it was identified that Ca and solubility was reflected in various factors such as chemical composition, shape and relative surface of HAP granules. However, Ca and P solubility did not always correlate with osteoconduction of HAP granules. While, a small amount of beta-TCP is producted in HAP when it is synthesized by precipitation mthod. Mg-contined HAP,however, made it possible to make statable bi-phase ceramics containing HAP and beta-TCP with sintering at 1200ﾟC.The effects of Mg-contained HAP and biocompatibility to osseous tissue were examined by using MC3T3-E1 cells derived from the neonatal mouse calvaria. As the result, the proliferation of MC3T3-E1 cells was promoted by immersing HAP into the medium. This assumed to indicate that HAP affects advantageously for activity of osteogenic cell. Furthermore, the proliferation of MC3T3-E1 cells was accelerated by Mg of the solution.

羟基磷灰石（HAP）在骨降低的各种特性中进行了合成的方法。表面表明YHE最出色的骨化性。在各种因素中，诸如HAP颗粒的形状和相对表面。通过使用源自新生儿小鼠钙的含量，将含有烧结的含量为HAP和beta-TCP，并使用MC3T3-E1细胞来检查对骨组织的生物兼容性。 。

项目成果

寺延 治: "人工骨としてのヒドロキシアパタイトの開発に関する研究" 神戸大学医学部神緑会学術誌. 9. 137-142 (1993)

Osamu Teranobe：“羟基磷灰石作为人造骨的开发研究”神户大学新绿会医学部杂志 9. 137-142 (1993)。

寺延 治: "人工骨としてのヒドロキシアパタイトの開発に関する研究" 神緑会学術誌. 9. 137-142 (1993)

Osamu Teranobe：“羟基磷灰石作为人造骨的开发研究”Shinryokukai学术杂志9. 137-142（1993）。

Isao Naito: "Cell Behavior of HAP Granule in Soft Tissue." Journal of Japanese Society of Oral Implantology. 7. 71-76 (1994)

Isao Naito：“HAP 颗粒在软组织中的细胞行为。”

内藤 勲: "軟組織中でのHAP顆粒周囲における細胞動態の検討" 日本口腔インプラント学会雑誌. 7. 71-76 (1994)

Isao Naito：“软组织中 HAP 颗粒周围的细胞动力学研究”日本口腔种植学会杂志 7. 71-76 (1994)。

Osamu Teranobu: "Study on Development of Hydoroxyapatite as Artificial Bone." Journal of Shinryokukai. 9. 137-142 (1993)

Osamu Teranobu：“羟基磷灰石作为人工骨的开发研究”。