Mitochondrial haplotypes and phenotypes in laying hens

蛋鸡线粒体单倍型和表型

• 批准号: 386177082
• 负责人: Professor Dr. Martin Hasselmann
• 金额: --
• 依托单位: Institut für Nutztierwissenschaften (460)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/386177082?language=en
• 关键词: Mitochondrial; haplotypes; phenotypes; laying; hens

Mitochondria are involved in several organismic key processes; hence the in-depth characterization of mtDNA haplotypes and mt gene expression in hens with efficient phosphorus (P) utilization and myo-inositol metabolism is of utmost importance. Myo-inositol is known to modulate mitochondrial functionality by so far unknown mechanisms related to autophagy and mitophagy. In the proposed project we will obtain whole mitochondrial genome haplotypes for two distinct strains of laying hens obtained in experiments of another project within the proposed Research Unit. This approach determines potential mutations that arose during the genetic selection process in laying hens affecting key enzyme activities. To test for mt gene expression differences among distinct haplotypes, tissue and developmental-specific quantitative (q) PCR experiments will be performed. We combine our data with those of genome analyses obtained in other projects of the Research Unit to find associations of interacting loci depending on the nuclear background. Further, mtDNA haplotypes are associated with the molecular information regarding metabolomics profiles, especially with acylcarnitines as indicators of mitochondrial function, assessed in another project. Thus, we will gain comprehensive insights into the functional importance of mitochondria in hens varying in phytate and P utilization and myo-inositol metabolism.

线粒体参与了几个有机的关键过程。因此，具有有效磷（P）利用率和肌醇代谢的母鸡中mtDNA单倍型和MT基因表达的深入表征至关重要。已知Myo-Insitol通过与自噬和线粒体有关的未知机制来调节线粒体功能。在拟议的项目中，我们将获得整个线粒体基因组单倍型，用于在拟议的研究单元中另一个项目的实验中获得的两种不同的分支母鸡。这种方法决定了在影响关键酶活性的母鸡的遗传选择过程中产生的潜在突变。为了测试不同单倍型之间的MT基因表达差异，将进行组织和发育特异性定量（Q）PCR实验。我们将数据与研究单元其他项目中获得的基因组分析相结合，以根据核背景找到相互作用的基因座的关联。此外，mtDNA单倍型与有关代谢组学特征的分子信息有关，尤其是在另一个项目中评估的酰基肉碱作为线粒体功能的指标。因此，我们将获得有关线粒体在植酸和P利用率以及肌醇代谢中的雌雄中的功能重要性的全面见解。