Microfluidic flow in printed fracture channel

打印裂缝通道中的微流体流动

• 批准号: 19F19329
• 负责人: Sivaniah Easan
• 金额: $ 1.41万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-10-11 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19329/
• 关键词: Paper submitted; Water flow in OM film; Electrophoresis; OM films on aluminium; Large image on OM film; Maskless printing of OM; Expanded film size of OM; Microfluidic flow in OM; Improved OM on plastic; Shared OM with community

In the final stages of the project, ending 2021, I began a collaboration with a Genetics research group to help develop the Organized Microfibrillation process for microfluidics. Up until this point I had difficulty getting water based solutions into the porous OM polymer films. Minor success was achieved with common additives and surfactants. With this new collaboration we were successfully able to identify some chemicals that enabled the flow of water based solutions in my OM channels. With this breakthrough, we were able to achieve other milestones with the OM films. Relevant biological materials, such as proteins and saccharides could enter the channels and could be tracked by fluorescent microscope. Finally we were able to achieve separation of biological materials with differing molecular weights, including separation of proteins and saccharaides, and separation of insulin and inert SARS-COV2 capsid. Separation was achieved by making OM channels with differing porosity such that larger molecules were blocked by the pores. These achievements have been included in a paper that was submitted to Nature Communications.With the same collaborators I also worked on how to move liquids through OM films without capillary action. A weakness of OM for microfluidics is the lack of pumping capability. During this time I tried applying electrophoresis to my OM films. I and my collaborators explored different experimental setups and conditions and were able to achieve electrophoretic flow preliminary experiments.

在该项目的最后阶段，即 2021 年结束时，我开始与遗传学研究小组合作，帮助开发微流体的有组织微纤维化过程。到目前为止，我很难将水基溶液注入多孔 OM 聚合物薄膜中。使用普通添加剂和表面活性剂取得了较小的成功。通过这次新的合作，我们成功地识别出了一些能够使水基溶液在我的 OM 通道中流动的化学物质。凭借这一突破，我们能够通过 OM 薄膜实现其他里程碑。相关生物材料，例如蛋白质和糖类可以进入通道并可以被荧光显微镜追踪。最终我们能够实现不同分子量生物材料的分离，包括蛋白质和糖类的分离，以及胰岛素和惰性SARS-COV2衣壳的分离。分离是通过制作具有不同孔隙率的 OM 通道来实现的，这样较大的分子就会被孔隙阻挡。这些成果已包含在提交给 Nature Communications 的论文中。我还与相同的合作者一起研究了如何在没有毛细管作用的情况下通过 OM 薄膜移动液体。 OM 对于微流体的一个弱点是缺乏泵送能力。在此期间，我尝试在 OM 胶片上应用电泳。我和我的合作者探索了不同的实验设置和条件，并能够实现电泳流初步实验。