Protein-protein interaction in the mammalian circadian system

哺乳动物昼夜节律系统中蛋白质-蛋白质相互作用

• 批准号: 35756735
• 负责人: Professor Dr. Achim Kramer
• 金额: --
• 依托单位: Fachbereich Chronobiologie (CVK)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/35756735?language=en
• 关键词: Protein; protein; interaction; mammalian; circadian

Circadian clocks are endogenous oscillators that regulate daily rhythms in physiology and behaviour. In almost every cell a gene-regulatory network generates molecular circadian oscillations with protein phosphorylation and complex formation playing a crucial role for their dynamics. Here, we will analyze these interactions (with PER2 as paradigmatic protein) both on the molecular and the functional level. To this end, we aim to identify kinases phosphorylating PER2, characterize its interactions with CRY1 and CKIε/δ as well as pharmacologically perturb these interactions in an established dynamical cell-culture model with subsequent analysis of the effect on circadian rhythms.

昼夜节律调节几乎每个细胞A-Regutory RK中的每日节奏的晶状体，都会产生蛋白质和compilex形成的分子振荡。为此，与CRY1和CKIε/δ相互作用以及药理学的磷酸化TS相互作用以及这些相互作用在已建立的动力学培养模型中，随后分析了对昼夜节律的影响。