Deep molecular characterization of eukaryotic microorganisms´ diversity and community composition in forest soils and the canopy region across biomes using a multiple barcoding approach - micDiv

使用多重条形码方法对森林土壤和生物群落冠层区域的真核微生物多样性和群落组成进行深入分子表征 - micDiv

• 批准号: 351263740
• 负责人: Professor Dr. Michael Bonkowski
• 金额: --
• 依托单位: AG Molekulare Evolution und Systematik der Tiere
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/351263740?language=en
• 关键词: Deep; molecular; characterization; eukaryotic; microorganisms

Soil protists occupy key nodes in terrestrial food webs due to their high abundance, fast turnover and functional importance as microbial grazers. However, methodological drawbacks in both culturing and molecular methods still strongly limit the knowledge of protist diversity, so that large groups remain virtually unknown. Accordingly, the structure of natural protist communities and taxa-specific ecological functions are largely unknown. We will use advanced cultivation-independent high throughput sequencing methods using group-specific primers and reverse rRNA-transcriptomics for a comprehensive assessment of protist diversity across all ecological compartments from forest soils (litter layer, mineral soil) to the canopy region (bark, leaves, dead wood, branch forks, knotholes, epiphytes) in temperate and tropical biomes.

土壤生物的占据陆地食物网中的关键节点，因为它们的丰度很高，流动迅速和作为微生物放牧者的功能重要性。但是，培养和分子方法中的方法论上的缺点仍然强烈限制了对素食者多样性的了解，因此大型群体几乎是未知的。因此，自然原生物群落和特异性生态功能的结构在很大程度上是未知的。 We will use advanced cultivation-independent high throughput sequencing methods using group-specific primers and reverse rRNA-transcriptomics for a comprehensive assessment of protist diversity across all ecological compartments from forest soils (litter layer, mineral soil) to the canopy region (bark, leaves, dead wood, branch forks, knotholes, epiphytes) in temperate and tropical biomes.