Zur Evolution der Larvenformen der Bivalvia (Mollusca): Ein high-tech-mikromorphologischer Vergleich

双壳纲（软体动物）幼虫形态的进化：高科技微形态比较

• 批准号: 34384108
• 负责人: Professor Dr. Gerhard Haszprunar
• 金额: --
• 依托单位: Zoologische Staatssammlung München
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/34384108?language=en
• 关键词: Zur; Evolution; der; Larvenformen; Bivalvia

The comparative-ontogenetic data on various molluscan groups, which have been generated by application of a broad methodology within the foregoing projects, should be widened concerning the protobranch Bivalvia. The remaining main topic is the clarification of the status of the pericalymma larva of the Protobranchia. Representatives of the respective taxa (mainly Nuculidae) will be collected alive and their larva will be bred. The various stages will be investigated by several high-tech morphological (light- and electron microscopy, computer-aided 3D-reconstruction, fluorescence labeling) methods. Objectives are: (1) Show the myo- und (2) in parts also neurogenesis of the different larval types by fluorescent dyes coupled with Confocal-Laser- Scanning-Microscopy (CLSM). Provide SEM/TEM-data on the (3) prototroch and on larval (4) sensory and (5) excretory organs and compare them with homologous structures in other molluscan classes. (6) Summarize the data to provide an evolutionary phylogeny (evo-devo) of Bivalvia and beyond. The main objectives have direct consequences for the bivalvian phylogeny and for a new framework about the origin and evolutionary history of the phylum Mollusca.

通过在上述项目中应用广泛的方法产生的各种软体动物类群的比较个体发育数据应该扩大到原分支双壳纲。剩下的主要话题是澄清原鳃纲周幼虫的地位。各个类群（主要是 Nuculidae）的代表将被活体收集并培育其幼虫。各个阶段将通过几种高科技形态学（光学和电子显微镜、计算机辅助 3D 重建、荧光标记）方法进行研究。目标是：（1）通过荧光染料结合共聚焦激光扫描显微镜（CLSM）显示不同幼虫类型的肌肉（2）部分神经发生。提供 (3) 原轮和幼虫 (4) 感觉器官和 (5) 排泄器官的 SEM/TEM 数据，并将它们与其他软体动物类别的同源结构进行比较。 (6) 总结数据以提供双壳纲及其他纲的进化系统发育 (evo-devo)。主要目标对双壳类系统发育和关于软体动物门起源和进化历史的新框架有直接影响。