Ecology and chemistry of plant-microbiome interactions in iron-deficient soil

缺铁土壤中植物-微生物组相互作用的生态学和化学

• 批准号: 20F20084
• 负责人: 東樹 宏和
• 金额: $ 1.79万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-09-25 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20F20084/
• 关键词: symbiosis; plants; mycorrhizal fungi; 共生; 土壌; 植物; microbiomes; chemical ecology; stable isotopes; nutrient defficiency

To finalize the research project, we have done the following experiments and analyses: 1) Experimental inoculation of endophytic fungi on model plant species: 2) Finalization of the tests for calibrating the necessary condition to successfully achieve stable isotope tracing techniques: these tests allowed us to characterize the necessary conditions to achieve measurable amounts of stable isotope tracer incorporated in different biomarkers for fungi and in plant biomass: 3) Carrying an in vitro experiment to test competition dynamics between different root endophytic fungi and arbuscular mycorrhizal fungi during nitrogen and carbon exchange with a model plant: this experiment allowed us to understand how endophytic fungi that carried similar function to AMF could modify the trading feature of carbon and nitrogen exchange in the AMF-plant symbiosis: 4) Carrying a pot experiment to validate the in vitro results: this experiment allowed us to generalize the findings obtained in in vitro conditions to soil-grown plants: 5) Transcriptomic analyses (RNA sequencing) of the model plant specimens used for the inoculation experiments of endophytic fungi. 6) In-silico analyses of the RNA-sequencing datasets mentioned above.

为了完成研究项目，我们做了以下实验和分析：1）内生真菌在模型植物物种上的实验接种：2）完成了校准成功实现稳定同位素示踪技术的必要条件的测试：这些测试使我们能够表征在不同的真菌生物标志物和植物生物量中获得可测量量的稳定同位素示踪剂的必要条件：3) 进行体外实验来测试不同根内生生物之间的竞争动态真菌和丛枝菌根真菌与模型植物进行氮和碳交换：该实验使我们能够了解具有与 AMF 类似功能的内生真菌如何改变 AMF-植物共生中碳和氮交换的交易特征：4）验证体外结果的盆栽实验：该实验使我们能够将在体外条件下获得的结果推广到土壤生长的植物：5）所用模型植物标本​​的转录组分析（RNA 测序）用于内生真菌的接种实验。 6) 对上述 RNA 测序数据集进行计算机分析。