分子認識PEGゲルキャピラリー電気泳動による生体分子の構造・サイズ選択的分離

通过分子识别 PEG 凝胶毛细管电泳对生物分子进行结构和尺寸选择性分离

• 批准号: 20J15151
• 负责人: 劉 晨晨
• 金额: $ 1.22万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-24 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20J15151/
• 关键词: poly(ethylene glycol); protein analysis; affinity electrophoresis; electrophoresis; DNA analysis

The poly(ethylene glycol) (PEG) based molecularly imprinted hydrogel (MIH) was fabricated for the molecular recognition of the target protein. Copoly(PEG acrylate/PEG diacrylate) (copoly(PEGA/PEGDA)) hydrogel was employed in the preparation of MIH for cytochrome c recognition. A highly specific molecular recognition was realized when using the MIH as a sorbent, evidenced by (1) the MIH showed adsorption to the cytochrome c target while the non-imprinted hydrogel showed no adsorption to the target, and (2) the MIH hydrogel showed higher adsorption to the cytochrome c target than to the other unspecific proteins. When tested as the affinity substance in electrophoresis, the MIH retarded the migration of cytochrome c to a higher extent compared with the other unspecific proteins.

为靶蛋白的分子识别制造了基于聚乙二醇（PEG）的分子印记水凝胶（MIH）。共同体（PEG丙烯酸酯/PEG二丙烯酸酯）（Copoly（PEGA/PEGDA））水凝胶用于MIH进行细胞色素C识别。当使用MIH用作吸附剂时，实现了高度特异性的分子识别，而（1）MIH证明了对细胞色素C靶标的吸附，而未刻印的水凝胶则没有表现出对靶标的吸附，并且（2）MIH水凝胶表现出对细胞色素c靶标的更高的吸附，而不是其他对其他cote nimspececific的吸附。与其他非特异性蛋白相比，当作为电泳中的亲和力物质测试时，细胞色素c的迁移程度更高。

项目成果

Study of Copoly(poly(ethylene glycol) diacrylate/poly(ethylene glycol) acrylate) Hydrogel as a Molecularly Imprinted Polymer for Cytochrome c Recognition

共聚(聚(乙二醇)二丙烯酸酯/聚(乙二醇)丙烯酸酯)水凝胶作为分子印迹聚合物用于细胞色素c识别的研究

• 发表时间: 2021
• 作者: Chenchen Liu;Takuya Kubo;Koji Otsuka
• 通讯作者: Koji Otsuka

Controllable molecular sieving effect in capillary electrophoresis by tuning the network structure of poly (ethylene glycol)-based hydrogels

通过调节聚乙二醇水凝胶的网络结构来控制毛细管电泳中的分子筛分效果

• 发表时间: 2021
• 作者: Chenchen Liu;Toyohiro Naito;Takuya Kubo;Koji Otsuka
• 通讯作者: Koji Otsuka

Controllable Molecular Sieving by copoly(Poly(ethylene glycol) Acrylate/Poly(ethylene glycol) Diacrylate)-Based Hydrogels via Capillary Electrophoresis for DNA Fragments

通过基于共聚（聚（乙二醇）丙烯酸酯/聚（乙二醇）二丙烯酸酯）的水凝胶通过毛细管电泳进行 DNA 片段的可控分子筛分

• DOI: 10.1021/acsapm.0c00567
• 发表时间: 2020-09-11
• 期刊: ACS APPLIED POLYMER MATERIALS
• 影响因子: 5
• 作者: Liu, Chenchen;Kubo, Takuya;Otsuka, Koji
• 通讯作者: Otsuka, Koji

Hydrogels in Electrophoresis: Applications and Advances

• DOI: 10.2116/analsci.20r004
• 发表时间: 2020-10
• 期刊: Analytical Sciences
• 影响因子: 1.6
• 作者: Chenchen Liu;T. Kubo;K. Otsuka

Online fluorescence imaging method by reducing the inequivalent photobleaching for quantitative capillary electrophoresis

减少不等量光漂白的在线荧光成像方法用于定量毛细管电泳

• DOI: 10.1016/j.snb.2020.128035
• 发表时间: 2020-09-15
• 期刊: SENSORS AND ACTUATORS B-CHEMICAL
• 影响因子: 8.4
• 作者: Liu, Chenchen;Yamaguchi, Yoshinori;Otsuka, Koji
• 通讯作者: Otsuka, Koji