The exploration of linking RNF20 depended histone H2B ubiquitylation and tumor suppressor gene p53 expression with inflammation and inflammation associated cancer

RNF20依赖性组蛋白H2B泛素化和抑癌基因p53表达与炎症及炎症相关癌症的关系探索

• 批准号: 319465792
• 负责人: Dr. Irina Kerle
• 金额: --
• 依托单位: Department of Molecular Cell Biology
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/319465792?language=en
• 关键词: exploration; linking; RNF20; depended; histone

In my short term fellowship at the laboratory of Professor Moshe Oren at the Department of Molecular Cell Biology of the Weizmann Institute in Rehovot, Israel, I will join a project linking the ligase RNF20 dependent ubiquitylation of histone H2B (H2Bub1) to inflammation and inflammation associated processes in colorectal cancerogenesis. By working with the knock out mouse model RNF20 +/- as well as p53 sequencing I will participate in the further examination of the inflammatory microenvironment and possible linkage of RNF20 depletion with the expression and mutation status of tumor suppressor gene p53. In order to examine the importance of these from mouse model and cell cultures derived findings for colitis and colorectal cancer in human patients I will examine the correlation of p53 expression and mutation status, H2Bub1 and cytokine levels like interleukine 6 and 8 in human colorectal tissue by establishing a H2Bub1 immunhistochemistry protocol and using qRT-PCR from tumor derived RNA of patient samples. My main goal is to learn new lab skills in a field thematically close to my doctoral thesis in a known and well-equipped laboratory such as the Oren lab. By learning these above mentioned lab skills and participating in the group´s performance of whole exome sequencing I will be able to expand my current research projects in the field of free floating tumor DNA detection in plasma and finding mutations that link different tumor entities and inflammatory processes.

在我在以色列Rehovot的Weizmann Institute分子细胞生物学系实验室的短期内奖学金中，我将加入一个将相关性H2B（H2BUB1）与炎症和炎症相关过程中的组蛋白H2B（H2BUB1）联系起来的依赖性泛素的项目。通过使用敲除小鼠模型RNF20 +/-以及p53测序，我将参与进一步检查炎症微环境和RNF20部署可能与肿瘤抑制基因p53的表达和突变状态的联系。为了检查人类患者中的小鼠模型和细胞培养物对结肠炎和结直肠癌的发现的重要性，我将检查p53表达和突变状态的相关性，H2BUB1和细胞因子水平（如白介碱6和8中）在人类结直肠组织中使用H2BUB1 Immunnunymistrys Protast和QRT-PCR来启用TUMOR SERNA，该水平6和8在人类结肠直肠组织中的相关性。我的主要目标是在一个知名且设备齐全的实验室（例如Oren Lab）中，在一个主题接近我博士学位的领域中学习新的实验室技能。通过学习上述实验室技能并参与整个外显子组测序的表现，我将能够在血浆中自由浮动肿瘤DNA检测领域扩展我当前的研究项目，并找到将不同肿瘤实体和炎症过程联系起来的突变。