Regulation of hemangioblst development by partial Epithelial Mesenchymal Transition

部分上皮间质转化对成血管细胞发育的调节

• 批准号: 21H02490
• 负责人: Sheng Guojun
• 金额: $ 11.15万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21H02490/
• 关键词: EMT; chicken; hemangioblast; hematopoiesis; vasculogenesis; Embryo

In FY2022, we have performed experiments on effect of overexpression of Snai2 and Zeb2 in lineage contribution (blood, vessel, smooth muscle lineages). We have found that these EMT markers do not clearly affect lineage marker gene expression. However, they affect cell lineage segregation, likely after lineage specification. We have also investigated relationship between hemangioblast specifiers (NPAS4L and ETV2, two markers upstream of blood/vessel markers SCL/LMO2 and smooth muscle markers HAND1/2) and EMT regulators. As EMT occurs both before (during mesoderm formation) and after hemagioblast specification and at least SNAI2 is involved in both primitive streak EMT and lateral plate mesoderm EMT (subject of this project), we are in the process of sorting out epistatic relationship between SNAI2, NPAS4L, and SCL/LMO2/HAND2).

在2022财年，我们对SNAI2和ZEB2在谱系贡献（血液，血管，平滑肌谱系）过表达的影响进行了实验。我们发现这些EMT标记并未明确影响谱系标记基因表达。但是，它们可能会在谱系规范之后影响细胞谱系分离。我们还研究了血管母细胞指数（NPAS4L和ETV2，两个上游血液/血管标志物SCL/LMO2和平滑肌标志物手动1/2）与EMT调节剂之间的关系。由于EMT在（在中胚层形成期间）和血质母细胞规范之后都发生，并且至少参与了原始条纹EMT和侧板中胚层EMT（该项目的主题），因此我们正在整理SNAI2，NPAS4L和SCL/LMO2/LMO2/HARD2）。

项目成果

Twenty Years on for The Epithelial-Mesenchymal Transition International Association (TEMTIA): An Interview with Co-Founders Erik Thompson and Donald Newgreen

国际上皮间充质转化协会 (TEMTIA) 二十周年：联合创始人埃里克·汤普森 (Erik Thompson) 和唐纳德·纽格林 (Donald Newgreen) 访谈

• DOI: 10.1159/000518250
• 发表时间: 2021
• 期刊: Cells Tissues Organs
• 影响因子: 2.7
• 作者: Sheng Guojun;Thompson Erik;Newgreen Donald;Denker Hans-Werner
• 通讯作者: Denker Hans-Werner

Special Issue: Epithelial-Mesenchymal Transition 2020

特刊：上皮-间质转化 2020

• DOI: 10.1159/000518686
• 发表时间: 2021
• 期刊: Cells Tissues Organs
• 影响因子: 2.7
• 作者: Thompson Erik W.;Sheng Guojun
• 通讯作者: Sheng Guojun

HMGN3 represses transcription of epithelial regulators to promote migration of cholangiocarcinoma in a SNAI2-dependent manner

• DOI: 10.1096/fj.202200386r
• 发表时间: 2022-07-01
• 期刊: FASEB JOURNAL
• 影响因子: 4.8
• 作者: Sorin, Supannika;Kubota, Sho;Sashida, Goro
• 通讯作者: Sashida, Goro

Inaugural Young Investigator Issue for Cells Tissues Organs

首届细胞、组织、器官青年研究者专刊

• DOI: 10.1159/000518410
• 发表时间: 2021
• 期刊: Cells Tissues Organs
• 影响因子: 2.7
• 作者: Gilbert Ryan J.;Sheng Guojun;Viebahn Christoph;Liebau Stefan;Marra Kacey G.;De Bartolo Loredana
• 通讯作者: De Bartolo Loredana

Kazan Federal University(ロシア連邦)

喀山联邦大学（俄罗斯联邦）