Needle-free microfluidic vaccine/drug delivery

无针微流控疫苗/药物输送

基本信息

批准号：
21H01253
负责人：
ホサノハミド
金额：
$ 10.57万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2021
资助国家：
日本
起止时间：
2021-04-01 至 2025-03-31
项目状态：
未结题

项目摘要

The goal of the research for year 2022 (R 4) was defined as investigation of nanosecond-pulsed-electric-field induced cavitation/shock wave streaming microjet; quantitative evaluation of in-vitro and ex-vivo deliveries; and publication.According to the plan, electrodes with minimize erosion and appropriate nozzle configuration for nanosecond pulse discharge microjet delivery were designed and tested. The ultra-high-speed real-time highly magnified optical setup, which was constructed last year, was used to evaluate the microfluidic and flow physics of the electric discharge induced microstreaming for different applied energies. A manuscript from results has been prepared for submission to a high impact journal. Experiments were performed to evaluate microjet penetration depth and volume in ex-vivo vaccine/drug deliveries. Experiments have been continued to conjugate new protein nanoparticles to minimize the drug/vaccine volume, a new manuscript from the results has been prepared.For quantitative evaluation, in-vitro and ex-vivo microscopic visualization of microjets in skin, soft tissue, and tumor models were performed. In-vivo experiments were performed to understand effects of microjet and shock waves microstreaming on cells. The interesting results was published in an article, additional manuscript has been prepared and is under submission. Histological evaluations of ex-vivo samples have been underway.

2022年的研究目标（R 4）被定义为纳秒脉冲电场诱导空化/冲击波流微射流的研究；体外和离体交付的定量评估；根据该计划，设计并测试了具有最小腐蚀的电极和用于纳秒脉冲放电微射流输送的适当喷嘴配置。去年建造的超高速实时高放大光学装置用于评估不同应用能量下放电引起的微流的微流体和流动物理特性。结果手稿已准备好提交给高影响力期刊。进行实验以评估离体疫苗/药物输送中的微射流穿透深度和体积。实验继续进行，以结合新的蛋白质纳米粒子，以最大限度地减少药物/疫苗的体积，根据结果准备了一份新的手稿。对于皮肤、软组织和肿瘤中微射流的定量评估、体外和离体显微可视化进行了模型。进行体内实验以了解微射流和冲击波微流对细胞的影响。有趣的结果发表在一篇文章中，额外的手稿已经准备好并正在提交。离体样本的组织学评估正在进行中。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Achieving non-invasive therapy and drug/vaccine delivery: Challenges and prospects

实现非侵入性治疗和药物/疫苗输送：挑战和前景

DOI：
发表时间：
2022
期刊：
影响因子：
0
作者：
Saho Ikeda;Masaya Hatanaka;Shinji Koganezawa;Shohei Kawada;Renguo Lu;Hiroshi Tani;Norio Tagawa;Hamid Hosano
通讯作者：
Hamid Hosano

Pulsed Power and Bioelectrics for Medial and Environmental Applications

用于医疗和环境应用的脉冲功率和生物电