Classification of HULIS carbon from different atmospheric environments via a 2-D-Off-line chromatography

通过二维离线色谱对来自不同大气环境的 HULIS 碳进行分类

• 批准号: 262792076
• 负责人: Professor Dr. Hartmut Herrmann
• 金额: --
• 依托单位: Leibniz-Institut für Troposphärenforschung e.V. (TROPOS)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/262792076?language=en
• 关键词: Classification; HULIS; carbon; different; atmospheric

Within the organic fraction of atmospheric particles, so-called "humic-like substances" (HULIS) make up a significant fraction. The chemical composition of this fraction is largely unknown. Separation techniques such as revered phase liquid chromatography (RP-LC) or capillary electrophoresis do not allow for a successful separation of this complex fraction. This is why the present projects suggests to apply a 2-dimensional separation based on polarity (RP-LC) and molecular size (size exclusion chromatography). The two dimensions (columns9 will be coupled offline and fractions will be collected to determine total organic carbon (TOC), UV-Vis absorbance, and elemental compositions of characteristic fragments by time-of-flight mass spectrometry (TOFMS). Particles from different environments will be sampled (European background, Asian megacity, rural location with strong biomass burning influence), in order to identify and compare patterns within the 2-dimensional polarity vs. size space. In addition, the results of the offline characterization will be compared with data from an online aerosol mass spectrometer. The results of this project are expected to allow for a better understanding of concentration, composition and possible sources of the important HULIS fraction of atmospheric particles.

在大气颗粒的有机部分中，所谓的“类腐殖质”（HULIS）占了很大一部分。该部分的化学成分很大程度上未知。反相液相色谱 (RP-LC) 或毛细管电泳等分离技术无法成功分离这种复杂的组分。这就是为什么当前的项目建议应用基于极性 (RP-LC) 和分子大小（尺寸排阻色谱）的二维分离。二维（第 9 列将离线耦合，并收集馏分，通过飞行时间质谱 (TOFMS) 确定总有机碳 (TOC)、紫外-可见光吸光度和特征碎片的元素组成。来自不同环境的颗粒将进行采样（欧洲背景、亚洲大城市、具有强烈生物质燃烧影响的农村地区），以便识别和比较二维极性与尺寸空间内的模式。此外，离线的结果。表征将与在线气溶胶质谱仪的数据进行比较。该项目的结果预计将有助于更好地了解大气颗粒物中重要 HULIS 部分的浓度、成分和可能来源。

项目成果

Two-Dimensional Offline Chromatographic Fractionation for the Characterization of Humic-Like Substances in Atmospheric Aerosol Particles.

• DOI: 10.1021/acs.est.7b00077
• 发表时间: 2017-04
• 期刊: Environmental science & technology
• 影响因子: 11.4
• 作者: T. Spranger;D. van Pinxteren;H. Herrmann