EAGER: APTAMER-INFUSED GRAPHENE TRANSISTOR TATTOOS AS TEMPORARY SWEAT BIOSENSING PLATFORM

EAGER：注入适体的石墨烯晶体管纹身作为临时汗液生物传感平台

• 批准号: 2400494
• 负责人: Dmitry Kireev
• 金额: $ 20万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2400494&HistoricalAwards=false
• 关键词: EAGER; APTAMER; INFUSED; GRAPHENE; TRANSISTOR

Sweat, a biofluid rich in essential bioanalytes, holds valuable insights into the physiological processes occurring within the human body. Current methods of biofluid testing, whether in sweat, blood, or interstitial fluid, are characterized by their time-consuming, invasive, and costly nature, rendering them impractical for regular use by the general population. The challenges associated with directing individuals to hospitals or clinics for continuous biofluid monitoring hinder the establishment of a comprehensive detection database and its correlation with behavioral patterns. One noteworthy example is the presence of cortisol in human biofluids, a biomarker associated with conditions such as stress, stroke, Cushing's syndrome, and Addison's disease. Despite its significance, existing technologies fall short in enabling regular intermittent monitoring of cortisol in sweat or other biofluids, especially in non-clinical, on-demand settings. Therefore, there is a compelling need to develop a novel modality that allows for superficial and user-friendly monitoring of the chemical composition of sweat, facilitating daily use by ordinary individuals. The impact of this research extends beyond technological innovation to encompass educational and societal dimensions. Graduate student training and the involvement of undergraduate students in the research process are integral components of this initiative. Additionally, leadership efforts in creating and spearheading programs that prepare undergraduates in biomedical engineering for graduate school contribute to goal of broadening participation within the field. Moreover, the dissemination of educational videos constitutes a proactive approach to increasing awareness and fostering interest in this interdisciplinary domain. These efforts collectively serve to bridge gaps between advanced technological research and the broader community, making the subject accessible and engaging to a diverse audience. The investigators propose to leverage the technology of graphene tattoos, by further functionalizing them with aptamers and bias as transistors directly via the skin. This way, the team will create the technology of aptamer-infused body-gated graphene tattoo transistors – a novel way to monitor one’s health. Such a sweat biosensing transistor tattoo will be biased through the body, via the unique skin-gating effect. The proof-of-concept devices will be designed to target cortisol, an important biomarker of stress, yet the technology will be multimodal and later can target detection of various biomolecules, cytokines, and viruses and be pivotal for future personalized healthcare. The investigators expect these sweat tattoo biosensing transistors to be self-administrable, allowing for non-clinical and on-demand biosensing, leading to frequent and even continuous monitoring of sweat bioanalytes. Beyond the task of frequent analyte monitoring, the technology holds the potential to improve the disease prognosis and prevention methodologies.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

汗液是一种富含必需生物分析物的生物液，对于人体内部发生的生理过程具有宝贵的见解，目前的生物液测试方法，无论是汗液、血液还是间质液，其特点是耗时、侵入性和成本高昂。的性质，使得它们对于普通人群的定期使用来说是不切实际的，指导个人到医院或诊所进行连续生物体液监测所面临的挑战阻碍了全面检测数据库及其与行为模式的相关性。人体生物体液中的皮质醇是一种与压力、中风、库欣综合征和阿狄森氏病等疾病相关的生物标志物，尽管其意义重大，但现有技术在定期间歇性监测汗液或其他生物体液中的皮质醇方面仍存在不足，尤其是在非临床领域。因此，迫切需要开发一种新的模式，可以对汗液的化学成分进行表面和用户友好的监测，以方便普通人的日常使用。这项研究的影响超出了范围。此外，涵盖教育和社会层面的技术创新和本科生参与研究过程是该计划的重要组成部分，领导层努力为本科生进入研究生院做好生物医学工程的准备。此外，传播教育视频是提高人们对该跨学科领域的认识和兴趣的积极方法，这些努力共同有助于弥合先进技术研究与更广泛社区之间的差距，使该主题易于理解和使用。吸引不同的受众。建议利用石墨烯纹身技术，通过适体和偏置直接通过皮肤将其进一步功能化为晶体管，这样，该团队将创建注入适体的体门控石墨烯纹身晶体管技术——一种监测人体的新方法。这种汗水生物传感晶体管纹身将通过独特的皮肤门控效应在身体中产生偏差。概念验证设备将针对皮质醇（压力的重要生物标志物）进行设计。该技术将是多模式的，以后可以针对各种生物分子、细胞因子和病毒的检测，并将成为未来个性化医疗保健的关键。研究人员预计这些汗液纹身生物传感晶体管可以自我管理，从而实现非临床和按需生物传感。 ，导致对汗液生物分析物进行频繁甚至连续的监测 除了频繁分析物监测的任务之外，该技术还具有改善疾病预后和预防方法的潜力。该奖项反映了 NSF 的法定规定。使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。