Collaborative Research: IIBR Instrumentation: A continuous metabolite sensor for lab and field studies

合作研究：IIBR Instrumentation：用于实验室和现场研究的连续代谢物传感器

• 批准号: 2324717
• 负责人: Allyson Hindle
• 金额: $ 23.08万
• 依托单位: University of Nevada Las Vegas
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-01 至 2027-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2324717&HistoricalAwards=false
• 关键词: Collaborative; Research; IIBR; Instrumentation; continuous

An award is made to the University of Nevada, Las Vegas to develop an implantable probe and recorder that continuously measures lactate in freely-moving wild animals and animals in the laboratory. Lactate plays many key roles in the body, including as a stress response signal, a fuel source, a signaling molecule and an indication of reaching maximum oxygen consumption during exercise or reaching the limit of aerobic diving in marine mammals. Despite its importance, lactate has never been continuously measured in freely-moving animals. With this device, which will also measure oxygen, glucose and temperature, researchers can continuously measure lactate and other variables in active, resting, diving or hibernating animals in the field or under controlled laboratory conditions. This new device will eliminate the impacts of having an investigator present since the implantable probe can be attached to a backpack recorder, allowing free movement of animals. The project will foster interdisciplinary collaborations between bioengineers and biologists from three different institutions and provide interdisciplinary scientific training for Ph.D. and undergraduate students and a postdoctoral fellow. Using the development of this device as a teaching tool, this project will provide unique training through a summer course on instrument development for underserved high school students in partnership with a non-profit organization. This innovation project will use specialized chemical formulations and light sensing technology to develop a 0.7 mm diameter, flexible probe that provides highly accurate, rapid and continuous measurements of key parameters, including lactate and glucose. Because each sensor is based on the same measurement design, users can choose sensors specific to their research questions, providing the flexibility required for a device that will be used by a wide-range of researchers. To ensure the probe’s function for different experiments or conditions, it will be validated to meet specific criteria for response time, accuracy, signal stability, sensitivity, precision and biocompatibility. It will also be tested in different animal models and environmental conditions to ensure its applicability to a diverse range of study sites, experiments and animal models. Continuous monitoring of oxygen and lactate levels will provide a clearer and more comprehensive picture of oxygen consumption, oxygen delivery to the tissues, lactate accumulation and lactate recovery in response to varied physiological challenges and stressors. This will be a major technological breakthrough for a number of NSF biology research communities, including physiology, ecology, energetics, conservation biology and exercise science.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

内华达大学拉斯维加斯分校获得了一项奖项，用于开发一种可植入的探针和记录仪，可以连续测量自由活动的野生动物和实验室动物的乳酸，乳酸在体内发挥着许多关键作用，包括作为应激反应信号。乳酸是一种燃料来源、一种信号分子，也是海洋哺乳动物运动时达到最大耗氧量或达到有氧潜水极限的指标，尽管它很重要，但从未使用该装置对自由活动的动物进行连续测量。还将测量氧气、葡萄糖和温度，研究人员可以在野外或受控实验室条件下连续测量活跃、休息、潜水或冬眠动物的乳酸和其他变量，这种新设备将消除研究人员在场的影响，因为植入式探针可以在场。连接到背包记录仪，允许动物自由移动，该项目将促进来自三个不同机构的生物工程师和生物学家之间的跨学科合作，并使用该设备的开发为博士生和本科生以及博士后研究员提供跨学科科学培训。作为教学该项目将与非营利组织合作，通过仪器开发暑期课程为服务不足的高中生提供独特的培训。该创新项目将使用专门的化学配方和光传感技术来开发直径 0.7 毫米的柔性探针。它可以对包括乳酸和葡萄糖在内的关键参数进行高精度、快速和连续的测量，因为每个传感器都基于相同的测量设计，因此用户可以根据其研究问题选择特定的传感器，从而为将要使用的设备提供所需的灵活性。由广泛的研究人员。探针在不同实验或条件下的功能，将被验证以满足响应时间、准确性、信号稳定性、灵敏度、精确度和生物相容性的特定标准，还将在不同的动物模型和环境条件下进行测试，以确保其适用于多种情况。一系列研究地点、实验和动物模型。持续监测氧气和乳酸水平将提供更清晰、更全面的氧气消耗、组织氧气输送、乳酸积累和乳酸恢复的情况，以应对各种生理挑战和压力。将对于许多 NSF 生物学研究界（包括生理学、生态学、能量学、保护生物学和运动科学）而言，这是一项重大技术突破。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查进行评估，被认为值得支持标准。