MRI: Track 1 Acquisition of a MALDI-TOF/TOF Mass Spectrometer for Research and Training at College of Charleston

MRI：轨道 1 采购 MALDI-TOF/TOF 质谱仪用于查尔斯顿学院的研究和培训

• 批准号: 2320174
• 负责人: Jay Forsythe
• 金额: $ 35万
• 依托单位: College of Charleston
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2320174&HistoricalAwards=false
• 关键词: MRI; Track; Acquisition; MALDI; TOF

This award is supported by the Major Research Instrumentation Program and the Chemistry Research Instrumentation Program. Professors Jay Forsythe, Michael Giuliano, Brooke Van Horn, Wendy Cory, and Yu Gong from College of Charleston will acquire a matrix-assisted laser desorption / ionization – tandem time of flight (MALDI-TOF/TOF) mass spectrometer. Mass spectrometry is a powerful technique to detect and characterize chemical species in sample mixtures. Specifically, MALDI-TOF/TOF mass spectrometry is well suited for analyzing molecules of numerous types – biological polymers, synthetic polymers / plastics, pharmaceuticals, dyes – and to provide essential information on their structural composition. This instrument will enable new research directions, encourage new collaborations, and boost research training not only for a diverse undergraduate student and researcher population at the College of Charleston but also for students and researchers across the Lowcountry region of South Carolina.This award is expected to enhance research and education throughout College of Charleston and beyond. MALDI-TOF mass spectrometry involves the conversion of chemical species to gas-phase ions that are accelerated through a field-free region under high vacuum. Ion mass-to-charge (m/z) values are then obtained from flight times. The instrument will provide greater than 10,000 resolving power (full width, half maximum) and low-ppm mass accuracy in reflector TOF mode; these capabilities are critical for a variety of research projects such as the fate(s) of pharmaceuticals in the environment, biocompatible materials, and the structure, function, and evolution of polypeptides. In TOF/TOF acquisition mode, ions in flight are selected and fragmented for structural analysis. Collisions with neutral gas such as argon increase the efficiency of fragmentation and, thus, the level of structural detail. Impacts of this award are far-reaching and include but are not limited to increasing participation of underrepresented persons in research through the Louis Stokes Alliance for Minority Participation (LS-AMP) program, better preparing students for future careers in clinical, industrial, or R&D labs by providing hands-on training with modern instrumentation, and access for other institutions in the region with no or limited mass spectrometry resources.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项得到了查尔斯顿学院重大研究仪器项目和化学研究仪器项目的​​支持，Jay Forsythe、Michael Giuliano、Brooke Van Horn、Wendy Cory 和 Yu Kong 教授将获得基质辅助激光解吸/电离串联装置。飞行时间 (MALDI-TOF/TOF) 质谱仪是一种检测和表征样品混合物中化学物质的强大技术。光谱分析非常适合分析多种类型的分子——生物聚合物、合成聚合物/塑料、药物、染料——并提供有关其结构组成的基本信息。该仪器将实现新的研究方向、鼓励新的合作并促进研究培训。不仅适用于查尔斯顿学院的多元化本科生和研究人员，也适用于南卡罗来纳州低地地区的学生和研究人员。该奖项预计将加强整个查尔斯顿学院及其他地区的研究和教育。转换然后，仪器将通过飞行时间获得在高真空下通过无场区域加速的化学物质到气相离子的离子质荷 (m/z) 值。反射器 TOF 模式下的功率（全宽度、半最大值）和低 ppm 质量精度；这些功能对于各种研究项目至关重要，例如药物在环境中的命运、生物相容性材料以及结构、功能，以及演化在 TOF/TOF 采集模式中，飞行中的离子被选择并碎片化，与中性气体（例如氩气）碰撞提高了碎片化的效率，因此，该奖项的结构细节水平具有深远的影响。包括但不限于通过路易斯斯托克斯少数民族参与联盟 (LS-AMP) 计划增加代表性不足的人对研究的参与，通过以下方式更好地为学生未来在临床、工业或研发实验室的职业做好准备提供现代仪器的实践培训，并为该地区没有或有限质谱资源的其他机构提供机会。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。