EAGER: APTAMER-INFUSED GRAPHENE TRANSISTOR TATTOOS AS TEMPORARY SWEAT BIOSENSING PLATFORM

EAGER：注入适体的石墨烯晶体管纹身作为临时汗液生物传感平台

• 批准号: 2400494
• 负责人: Dmitry Kireev
• 金额: $ 20万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2400494&HistoricalAwards=false
• 关键词: EAGER; APTAMER; INFUSED; GRAPHENE; TRANSISTOR

Sweat, a biofluid rich in essential bioanalytes, holds valuable insights into the physiological processes occurring within the human body. Current methods of biofluid testing, whether in sweat, blood, or interstitial fluid, are characterized by their time-consuming, invasive, and costly nature, rendering them impractical for regular use by the general population. The challenges associated with directing individuals to hospitals or clinics for continuous biofluid monitoring hinder the establishment of a comprehensive detection database and its correlation with behavioral patterns. One noteworthy example is the presence of cortisol in human biofluids, a biomarker associated with conditions such as stress, stroke, Cushing's syndrome, and Addison's disease. Despite its significance, existing technologies fall short in enabling regular intermittent monitoring of cortisol in sweat or other biofluids, especially in non-clinical, on-demand settings. Therefore, there is a compelling need to develop a novel modality that allows for superficial and user-friendly monitoring of the chemical composition of sweat, facilitating daily use by ordinary individuals. The impact of this research extends beyond technological innovation to encompass educational and societal dimensions. Graduate student training and the involvement of undergraduate students in the research process are integral components of this initiative. Additionally, leadership efforts in creating and spearheading programs that prepare undergraduates in biomedical engineering for graduate school contribute to goal of broadening participation within the field. Moreover, the dissemination of educational videos constitutes a proactive approach to increasing awareness and fostering interest in this interdisciplinary domain. These efforts collectively serve to bridge gaps between advanced technological research and the broader community, making the subject accessible and engaging to a diverse audience. The investigators propose to leverage the technology of graphene tattoos, by further functionalizing them with aptamers and bias as transistors directly via the skin. This way, the team will create the technology of aptamer-infused body-gated graphene tattoo transistors – a novel way to monitor one’s health. Such a sweat biosensing transistor tattoo will be biased through the body, via the unique skin-gating effect. The proof-of-concept devices will be designed to target cortisol, an important biomarker of stress, yet the technology will be multimodal and later can target detection of various biomolecules, cytokines, and viruses and be pivotal for future personalized healthcare. The investigators expect these sweat tattoo biosensing transistors to be self-administrable, allowing for non-clinical and on-demand biosensing, leading to frequent and even continuous monitoring of sweat bioanalytes. Beyond the task of frequent analyte monitoring, the technology holds the potential to improve the disease prognosis and prevention methodologies.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

汗水是一种富含基本生物分析的生物流体，对人体内部发生的物理过程具有宝贵的见解。当前的生物流体测试方法，无论是在汗，血液还是间质性液中，都以其耗时，侵入性和昂贵的性质为特征，使其不切实际，以便普通人群的定期使用。与将个人指向医院或诊所有关的挑战连续生物流体监测阻碍了全面检测数据库的建立及其与行为模式的相关性。一个值得注意的例子是人类生物流体中存在皮质醇，这是一种与压力，中风，库欣综合症和艾迪生氏病有关的生物标志物。尽管具有重要意义，但现有技术在实现汗水或其他生物流体中的皮质醇的定期间歇性监测方面缺乏，尤其是在非临床的，点播的环境中。因此，有迫切需要开发一种新型的方式，该方式可以允许对汗水的化学成分进行表面和用户友好的监测，从而支持普通人的日常使用。这项研究的影响扩展了技术创新，以涵盖教育和社会层面。研究生培训和本科生参与研究过程的参与是该计划不可或缺的组成部分。此外，领导努力在创建和率领计划的计划中为研究生院的生物医学工程准备的本科生做好了贡献，这有助于扩大该领域的参与。此外，教育视频的传播构成了一种积极的方法，可以提高意识并促进对这个跨学科领域的兴趣。这些努力共同为高级技术研究与更广泛的社区之间的差距弥合了差距，使该主题可以访问并吸引潜水员受众。研究人员建议通过直接通过皮肤直接将其作为晶体管来进一步使它们官能化，以利用石墨烯纹身的技术。这样，团队将创建适体注射的石墨烯纹身晶体管的技术，这是监测健康的新方法。这种汗水的生物传感晶体管纹身会通过独特的皮肤门控作用偏向人体。概念验证设备将旨在靶向皮质醇，这是一种重要的压力生物标志物，但是该技术将是多模式的，后来可以针对各种生物分子，细胞因子和病毒的检测，并且是未来个性化医疗保健的关键。研究人员期望这些汗纹身生物传感晶体管具有自给自足，从而可以进行非临床和按需生物传感，从而经常甚至继续监测汗水生物分析。除了经常监测的任务外，该技术还具有改善疾病进展和预防方法的潜力。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子优点和更广泛的影响评估标准来评估，被认为是珍贵的支持。