FMSG: Bio: RAMP-Bio-CAFe: Routing Advanced Manufacturing of BioPolymer(s): Capturing Agri-feedstocks in Cell free Factories

FMSG：生物：RAMP-Bio-CAFe：生物聚合物的先进制造路线：在无细胞工厂中捕获农业原料

• 批准号: 2328291
• 负责人: TANVI GOVIL
• 金额: $ 50万
• 依托单位: South Dakota School of Mines and Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-01 至 2025-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2328291&HistoricalAwards=false
• 关键词: FMSG; Bio; RAMP; CAFe; Routing

Bacteria produce polymers when there is excess carbon. Bacterial carbon storage polymers are referred to as PHAs. PHAs are biodegradable and exhibit useful material properties. Recovering PHAs from bacteria is a major hurdle. The primary objective of the project is to develop a cell-free method for PHA synthesis. This would greatly simplify the recovery process and would increase the yield of the polymer from natural feedstocks. Enzymes from a novel thermophilic bacteria will perform the cell-free synthesis. The productivity of that system will be characterized. Undergraduates from nearby tribal colleges will be recruited to participate in the research. They will be supported by a series of professional development online workshops developed as part of this project.This project will establish a proof-of-concept to rewire the synthesis of PHAs as an extracellular product. To accomplish this, several intermediate steps are anticipated. First is the single-step consolidated bioconversion of agri-materials into PHA precursors by engineering a non-model microbial strain to synthesize C6-C12 hydroxy acids from unprocessed corn stover. Next is cell-free enzymatic polymerization of these acids into PHA using thermostable enzymes from the model strain. Finally, purification of the synthesized biopolymers using green ionic liquids.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

当碳过多时，细菌会产生聚合物。细菌碳储存聚合物称为PHA。 PHA是可生物降解的，并且具有有用的材料特性。从细菌中恢复PHA是一个主要障碍。该项目的主要目的是开发一种无细胞合成的方法。这将极大地简化恢复过程，并会增加自然原料的聚合物的产量。来自新型嗜热细菌的酶将执行无细胞的合成。该系统的生产率将被表征。附近部落学院的本科生将被招募参加研究。作为该项目的一部分开发的一系列专业发展在线研讨会，将为他们提供支持。本项目将建立概念验证，以重新连接PHAS作为细胞外产品。为此，预计会有几个中间步骤。首先是通过设计非模型微生物菌株来合成未经处理的玉米毒剂的C6-C12羟基酸，将农业材料的单步合并生物转化为PHA前体。接下来是使用模型应变的热稳定酶将这些酸无细胞的酶聚合分解为PHA。最后，使用绿色离子液体对合成的生物聚合物进行纯化。该奖项反映了NSF的法定任务，并使用基金会的知识分子优点和更广泛的影响审查标准，被认为值得通过评估来获得支持。